ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MESCDCFETH VNQDDLLVKY QYISDALIAL AYFSIPLELI YFVQKSAFFP YKWVLMQFGA 

        70         80         90        100        110        120 
FIILCGATHF INLWMFFMHS KAVAIVMTIA KVSCAVVSCA TALMLVHIIP DLLSVKNREL 

       130        140        150        160        170        180 
FLKKKADELD REMGLILTQE ETGRHVRMLT HGIRRTLDRH TILRTTLVEL GKTLCLEECA 

       190        200        210        220        230        240 
LWMPSQSGLY LQLSHTLSHK IQVGSSVPIN LPIINELFNS AQAMHIPHSC PLAKIGPPVG 

       250        260        270        280        290        300 
RYSPPEVVSV RVPLLHLSNF QGSDWSDLSG KGYAIMVLIL PTDGARKWRD HELELVENVA 

       310        320        330        340        350        360 
DQVAVALSHA AILEESMHAR DQLMEQNFAL DKARQEAEMA VHARNDFLAV MNHEMRTPMH 

       370        380        390        400        410        420 
AIISLSSLLL ETELSPEQRV MIETILKSSN LVATLISDVL DLSRLEDGSL LLENEPFSLQ 

       430        440        450        460        470        480 
AIFEEVISLI KPIASVKKLS TNLILSADLP TYAIGDEKRL MQTILNIMGN AVKFTKEGYI 

       490        500        510        520        530        540 
SIIASIMKPE SLQELPSPEF FPVLSDSHFY LCVQVKDTGC GIHTQDIPLL FTKFVQPRTG 

       550        560        570        580        590        600 
TQRNHSGGGL GLALCKRFVG LMGGYMWIES EGLEKGCTAS FIIRLGICNG PSSSSGSMAL 

       610 
HLAAKSQTRP WNW 

References and documentation are available.

Number of amino acids: 613

Molecular weight: 68332.73

Theoretical pI: 6.06

Amino acid composition: 
Ala (A)  44	  7.2%
Arg (R)  24	  3.9%
Asn (N)  19	  3.1%
Asp (D)  24	  3.9%
Cys (C)  13	  2.1%
Gln (Q)  23	  3.8%
Glu (E)  39	  6.4%
Gly (G)  33	  5.4%
His (H)  22	  3.6%
Ile (I)  46	  7.5%
Leu (L)  82	 13.4%
Lys (K)  28	  4.6%
Met (M)  24	  3.9%
Phe (F)  25	  4.1%
Pro (P)  27	  4.4%
Ser (S)  53	  8.6%
Thr (T)  29	  4.7%
Trp (W)   8	  1.3%
Tyr (Y)  12	  2.0%
Val (V)  38	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      3072
Hydrogen    H	      4891
Nitrogen    N	       807
Oxygen      O	       876
Sulfur      S	        37

Formula: C3072H4891N807O876S37
Total number of atoms: 9683

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    62630
Abs 0.1% (=1 g/l)   0.917, assuming all pairs of Cys residues form cystines


Ext. coefficient    61880
Abs 0.1% (=1 g/l)   0.906, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.90
This classifies the protein as unstable.



Aliphatic index: 106.59

Grand average of hydropathicity (GRAVY): 0.177