ProtParam
User-provided sequence:
10 20 30 40 50 60
MESCFPAMKL NQCTFGLNNE IVSERVSAFW GTQVVKPNHL RTTKLRSAPQ KKIQTNLIRS
70 80 90 100 110 120
VLTPFVDQES HEPLLRTQNA DPKNVASIIL GGGAGTRLFP LTSKRAKPAV PIGGCYRLID
130 140 150 160 170 180
IPMSNCINSG IRKIFILTQF NSFSLNRHLS RTYNFGNGVN FGDGFVEVLA ATQTSGDAGK
190 200 210 220 230 240
KWFQGTADAV RQFIWVFEDA KTKNVEHVLI LSGDHLYRMD YMNFVQKHIE SNADITVSCL
250 260 270 280 290 300
PMDESRASDF GLLKIDQSGK IIQFSEKPKG DDLKAMQVDT SILGLPPKEA AESPYIASMG
310 320 330 340 350 360
VYVFRKEVLL KLLRSSYPTS NDFGSEIIPL AVGEHNVQAF LFNDYWEDIG TIGSFFDANL
370 380 390 400 410 420
ALTEQPPKFQ FYDQKTPFFT SPRFLPPTKV DKCRILDSIV SHGCFLRECS VQHSIVGIRS
430 440 450 460 470 480
RLESGVELQD TMMMGADFYQ TEAEIASLLA EGKVPVGVGQ NTKIKNCIID KNAKIGKNVV
490 500 510
IANADGVEEG DRPEEGFHIR SGITVVLKNA TIRDGLHI
References and
documentation are available.
Number of amino acids: 518
Molecular weight: 57469.81
Theoretical pI: 7.15
Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 57
Atomic composition:
Carbon C 2568
Hydrogen H 4048
Nitrogen N 698
Oxygen O 758
Sulfur S 20
Formula: C
2568H
4048N
698O
758S
20
Total number of atoms: 8092
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 37400
Abs 0.1% (=1 g/l) 0.651, assuming all pairs of Cys residues form cystines
Ext. coefficient 36900
Abs 0.1% (=1 g/l) 0.642, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.06
This classifies the protein as stable.
Aliphatic index: 87.32
Grand average of hydropathicity (GRAVY): -0.176