ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MESEAIQEDL QNLDVELKDV QGQISALIEH QDRLYERKSE LKTLLKALAA SGSPVASSGG 

        70         80         90        100        110        120 
SSAIENWSET FEWDSRADDV RFNVFGISKY RANQKEIINA IMTGRDVLVI MAAGGGKSLC 

       130        140        150        160        170        180 
YQLPAMLRGG TTLVVSPLLS LIQDQVMGLA ALGISAYMLT STSGKENEKF VYKALEKGED 

       190        200        210        220        230        240 
DLKILYVTPE KVSKSKRFMS KLEKCHNAGR LSLISIDEAH CCSQWGHDFR PDYKNLSILK 

       250        260        270        280        290        300 
TQFPKVPMVA LTATATQKVQ NDLIEMLHIP KCVKFVSSVN RPNLFYSVRE KSAVGKLVVD 

       310        320        330        340        350        360 
EIAEFIRESY SNNESGIVYC FSRKECEQIA GDLRERGISA DYYHADMDAN MREKVHMRWS 

       370        380        390        400        410        420 
KNKLQVIVGT VAFGMGINKP DVRFVIHHSL SKSMETYYQE SGRAGRDGLP SECILFFRSA 

       430        440        450        460        470        480 
DVPRQSSMVF YEYSGLQNLY DIVRYCQSKT KCRRSAFFRH FGEPSQDCNG MCDNCALSSE 

       490        500        510        520        530        540 
VKEVDVSDLS KLVVSMVQET QAKDQRVTML QLGDKLRNKH KDLIAELKRD EVEHLVIKLI 

       550        560        570        580        590        600 
VDSVLKEEFQ HTPYSTNAYV TMGPLANQLL QGRKTIKMET SSRQTKKLKR SITFSGLELK 

       610        620        630        640        650        660 
LDELRKEISA ADGSILPHTV LSTQQIGSIS SQKPVSLQEL ESIIGKLKTE KYGDRILEEV 

       670        680        690        700 
MRHEAVSEQL VEDPTKEETC KSRLRKRAKT QKDVVLVESS GEEEA 

References and documentation are available.

Number of amino acids: 705

Molecular weight: 79368.84

Theoretical pI: 7.21

Amino acid composition: 
Ala (A)  42	  6.0%
Arg (R)  39	  5.5%
Asn (N)  22	  3.1%
Asp (D)  38	  5.4%
Cys (C)  14	  2.0%
Gln (Q)  34	  4.8%
Glu (E)  59	  8.4%
Gly (G)  39	  5.5%
His (H)  15	  2.1%
Ile (I)  39	  5.5%
Leu (L)  68	  9.6%
Lys (K)  58	  8.2%
Met (M)  21	  3.0%
Phe (F)  21	  3.0%
Pro (P)  18	  2.6%
Ser (S)  70	  9.9%
Thr (T)  30	  4.3%
Trp (W)   4	  0.6%
Tyr (Y)  21	  3.0%
Val (V)  53	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 97
Total number of positively charged residues (Arg + Lys): 97

Atomic composition:

Carbon      C	      3477
Hydrogen    H	      5622
Nitrogen    N	       970
Oxygen      O	      1077
Sulfur      S	        35

Formula: C3477H5622N970O1077S35
Total number of atoms: 11181

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54165
Abs 0.1% (=1 g/l)   0.682, assuming all pairs of Cys residues form cystines


Ext. coefficient    53290
Abs 0.1% (=1 g/l)   0.671, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.60
This classifies the protein as unstable.



Aliphatic index: 86.95

Grand average of hydropathicity (GRAVY): -0.385