ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MESGSMKTPL VNNQEEARSS SSITCGLLLS TSVAVTGSFV YGCAMSYSSP AQSKIMEELG 

        70         80         90        100        110        120 
LSVADYSFFT SVMTLGGMIT AAFSGKIAAV IGRRQTMWIA DVFCIFGWLA VAFAHDKMLL 

       130        140        150        160        170        180 
NIGRGFLGFG VGLISYVVPV YIAEITPKAF RGGFSFSNQL LQSFGISLMF FTGNFFHWRT 

       190        200        210        220        230        240 
LALLSAIPCG IQMICLFFIP ESPRWLAMYG RERELEVTLK RLRGENGDIL EEAAEIRETV 

       250        260        270        280        290        300 
ETSRRESRSG LKDLFNMKNA HPLIIGLGLM LLQQFCGSSA ISAYAARIFD TAGFPSDIGT 

       310        320        330        340        350        360 
SILAVILVPQ SIIVMFAVDR CGRRPLLMSS SIGLCICSFL IGLSYYLQNH GDFQEFCSPI 

       370        380        390        400        410        420 
LIVGLVGYVL SFGIGLGGLP WVIMSEVFPV NVKITAGSLV TVSNWFFSWI IIFSFNFMMQ 

       430        440        450        460 
WSAFGTYFIF AGVSLMSFVF VWTLVPETKG RTLEDIQQSL GQLS 

References and documentation are available.

Number of amino acids: 464

Molecular weight: 50965.67

Theoretical pI: 6.45

Amino acid composition: 
Ala (A)  31	  6.7%
Arg (R)  20	  4.3%
Asn (N)  12	  2.6%
Asp (D)  10	  2.2%
Cys (C)  10	  2.2%
Gln (Q)  15	  3.2%
Glu (E)  21	  4.5%
Gly (G)  44	  9.5%
His (H)   4	  0.9%
Ile (I)  40	  8.6%
Leu (L)  50	 10.8%
Lys (K)  10	  2.2%
Met (M)  19	  4.1%
Phe (F)  39	  8.4%
Pro (P)  15	  3.2%
Ser (S)  50	 10.8%
Thr (T)  22	  4.7%
Trp (W)   9	  1.9%
Tyr (Y)  11	  2.4%
Val (V)  32	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      2340
Hydrogen    H	      3614
Nitrogen    N	       578
Oxygen      O	       637
Sulfur      S	        29

Formula: C2340H3614N578O637S29
Total number of atoms: 7198

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    66515
Abs 0.1% (=1 g/l)   1.305, assuming all pairs of Cys residues form cystines


Ext. coefficient    65890
Abs 0.1% (=1 g/l)   1.293, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.80
This classifies the protein as unstable.



Aliphatic index: 102.33

Grand average of hydropathicity (GRAVY): 0.574