ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MESKEISIRS RTPPSKLYSI QSCIGRGNFG DVYKAVDRVT QEIVAIKVVN LEHSDEDIEL 

        70         80         90        100        110        120 
LAQEIFFLAE LKSPLITNYI ATMLEDVSMW IVMEYCGGGS CSDLLKRSYV NGLPEEKVSF 

       130        140        150        160        170        180 
IIHEVTLGLK YLHEQRKIHR DIKAANILLN EEGMVKLGDF GVSGHIRSTL KRDTFVGTPY 

       190        200        210        220        230        240 
WMAPEVVCCE VDGYNEKADI WSLGITTYEL LKGLPPLSKY DPMKVMTNLP KRKPPKLQGP 

       250        260        270        280        290        300 
FSDAAKDFVA GCLVKTPADR PSAYNLLSFE FVKNITITNL KSDVDLIKQK KVQERYTKVP 

       310        320        330        340        350        360 
KYPLQNRLYK NSNTVRGKEF WNFESTRLST TQISKEELSP ITQDSPTSSL NMESPYLLHG 

       370        380        390        400        410        420 
QTVTPITNPS SSSFRKCTQP VFELDSGMDI DSGCPNAQAE TEIVPLSNHN KKHKKNDIQA 

       430        440        450        460        470        480 
LKIEKFDYLK NIVSHILNRM YDRARDDETR KYVNEMLKQF IKTEANVPGF NEVFIEEISL 

       490 
RIEAIKKGFV 

References and documentation are available.

Number of amino acids: 490

Molecular weight: 55704.01

Theoretical pI: 7.55

Amino acid composition: 
Ala (A)  20	  4.1%
Arg (R)  21	  4.3%
Asn (N)  26	  5.3%
Asp (D)  26	  5.3%
Cys (C)   8	  1.6%
Gln (Q)  15	  3.1%
Glu (E)  38	  7.8%
Gly (G)  24	  4.9%
His (H)   9	  1.8%
Ile (I)  36	  7.3%
Leu (L)  44	  9.0%
Lys (K)  44	  9.0%
Met (M)  12	  2.4%
Phe (F)  19	  3.9%
Pro (P)  26	  5.3%
Ser (S)  39	  8.0%
Thr (T)  28	  5.7%
Trp (W)   4	  0.8%
Tyr (Y)  18	  3.7%
Val (V)  33	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 65

Atomic composition:

Carbon      C	      2490
Hydrogen    H	      3960
Nitrogen    N	       660
Oxygen      O	       745
Sulfur      S	        20

Formula: C2490H3960N660O745S20
Total number of atoms: 7875

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49320
Abs 0.1% (=1 g/l)   0.885, assuming all pairs of Cys residues form cystines


Ext. coefficient    48820
Abs 0.1% (=1 g/l)   0.876, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.05
This classifies the protein as unstable.



Aliphatic index: 87.29

Grand average of hydropathicity (GRAVY): -0.391