ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MESLPTTVSG KSDRRGRFSK SQNTSKPSLI LAFFSCLAWL YVAGRLWQDA QYRAALNTVL 

        70         80         90        100        110        120 
KMNYDQRPKV LTVEDKLVVL GCKDLERRIV ETEMELAQAK SQGYLKKQKS VSSSGKKMLA 

       130        140        150        160        170        180 
VIGVYTGFGS HLKRNKFRGS WMPRDDALKK LEERGVVIRF VIGRSANRGD SLDRKIDEEN 

       190        200        210        220        230        240 
RATKDFLILE NHEEAQEELP KKVKFFYSAA VQNWDAEFYV KVDDNVDLDL EGMIALLESR 

       250        260        270        280        290        300 
RSQDGAYIGC MKSGDVITEE GSQWYEPEWW KFGDDKSYFR HATGSLVILS KNLAQYVNIN 

       310        320        330        340 
SGLLKTYAFD DTTIGSWMIG VQATYIDDNR LCCSSTRQEK VCSMA 

References and documentation are available.

Number of amino acids: 345

Molecular weight: 39271.73

Theoretical pI: 8.55

Amino acid composition: 
Ala (A)  23	  6.7%
Arg (R)  22	  6.4%
Asn (N)  13	  3.8%
Asp (D)  24	  7.0%
Cys (C)   6	  1.7%
Gln (Q)  14	  4.1%
Glu (E)  23	  6.7%
Gly (G)  23	  6.7%
His (H)   3	  0.9%
Ile (I)  15	  4.3%
Leu (L)  32	  9.3%
Lys (K)  29	  8.4%
Met (M)   9	  2.6%
Phe (F)  13	  3.8%
Pro (P)   6	  1.7%
Ser (S)  30	  8.7%
Thr (T)  15	  4.3%
Trp (W)   8	  2.3%
Tyr (Y)  13	  3.8%
Val (V)  24	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 51

Atomic composition:

Carbon      C	      1739
Hydrogen    H	      2745
Nitrogen    N	       481
Oxygen      O	       525
Sulfur      S	        15

Formula: C1739H2745N481O525S15
Total number of atoms: 5505

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63745
Abs 0.1% (=1 g/l)   1.623, assuming all pairs of Cys residues form cystines


Ext. coefficient    63370
Abs 0.1% (=1 g/l)   1.614, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.76
This classifies the protein as unstable.



Aliphatic index: 79.97

Grand average of hydropathicity (GRAVY): -0.459