ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MESMTPISCP TTPRWNQDRP FLTGRFHQET RASSKFADSK RFTLDSSSSG VEQAIGCYDT 

        70         80         90        100        110        120 
PVQELIVIDD LLSALVGIEG RYISIKRFHG KEDSIAFQVD PSMDLALQEL AKRIFPLCEY 

       130        140        150        160        170        180 
YLLIDQFVES SSQFKNGLVN HAFAAALRAL LLDYQAMVAQ LEHQFRLGRL SIQGLWFYCQ 

       190        200        210        220        230        240 
PMMGSMRALA AVIQQASTKQ FVGSGVLNLL QSQAKAMAGD NSVRSLLEKM TECASNAYLS 

       250        260        270        280        290        300 
ILERWVYEGI IDDPYGEFFI AENRSLKKES LSQDSTAKYW SQRYSLKDTI PGFLANIAAT 

       310        320        330        340        350        360 
ILTTGKYLNV MRECGHNVQV PISERSKLTI FGSNHHYLEC IKAAHEFASI ELVNLIKDKY 

       370        380        390        400        410        420 
DLVGRLRSIK HYLLLDQGDF LVHFMDIARE ELNKKVHEIS VEKLQSLLDL ALRTTAAAAD 

       430        440        450        460        470        480 
PRHEDLTCCV DRASLLTTLG MHKDTDSNSI EDPMSITGLE TFSLSYKVQW PLSIVISKKA 

       490        500        510        520        530        540 
LSKYQLIFRF LFHCKHVERQ LCGAWQIHQG IRSMNSKGTA ILRSSLLCRS MLKFISSLLH 

       550        560        570        580        590        600 
YLTFEVLEPN WHVMHDRLQS TRSVDEVIQH HDFFLDKCLR GCLLLLPDVL KKMEKLKSVC 

       610        620        630        640        650        660 
LQYAAATQWL ISSSIDINSQ SHPQKTMIRD TTVTESIFNF EREFNSELQS LGPVLSKGSQ 

       670 
AEPYLTHLSQ WILGVSKE 

References and documentation are available.

Number of amino acids: 678

Molecular weight: 76945.55

Theoretical pI: 6.77

Amino acid composition: 
Ala (A)  45	  6.6%
Arg (R)  34	  5.0%
Asn (N)  19	  2.8%
Asp (D)  35	  5.2%
Cys (C)  15	  2.2%
Gln (Q)  37	  5.5%
Glu (E)  40	  5.9%
Gly (G)  30	  4.4%
His (H)  23	  3.4%
Ile (I)  43	  6.3%
Leu (L)  87	 12.8%
Lys (K)  38	  5.6%
Met (M)  18	  2.7%
Phe (F)  31	  4.6%
Pro (P)  19	  2.8%
Ser (S)  69	 10.2%
Thr (T)  32	  4.7%
Trp (W)   9	  1.3%
Tyr (Y)  20	  2.9%
Val (V)  34	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      3439
Hydrogen    H	      5431
Nitrogen    N	       929
Oxygen      O	      1006
Sulfur      S	        33

Formula: C3439H5431N929O1006S33
Total number of atoms: 10838

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    80175
Abs 0.1% (=1 g/l)   1.042, assuming all pairs of Cys residues form cystines


Ext. coefficient    79300
Abs 0.1% (=1 g/l)   1.031, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.11
This classifies the protein as unstable.



Aliphatic index: 95.96

Grand average of hydropathicity (GRAVY): -0.119