ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MESNCVQFLG NKTILITGAP GFLAKVLVEK ILRLQPNVKK IYLLLRAPDE KSAMQRLRSE 

        70         80         90        100        110        120 
VMEIDLFKVL RNNLGEDNLN ALMREKIVPV PGDISIDNLG LKDTDLIQRM WSEIDIIINI 

       130        140        150        160        170        180 
AATTNFDERY DIGLGINTFG ALNVLNFAKK CVKGQLLLHV STAYISGEQP GLLLEKPFKM 

       190        200        210        220        230        240 
GETLSGDREL DINIEHDLMK QKLKELQDCS DEEISQTMKD FGMARAKLHG WPNTYVFTKA 

       250        260        270        280        290        300 
MGEMLMGKYR ENLPLVIIRP TMITSTIAEP FPGWIEGLKT LDSVIVAYGK GRLKCFLADS 

       310        320        330        340        350        360 
NSVFDLIPAD MVVNAMVAAA TAHSGDTGIQ AIYHVGSSCK NPVTFGQLHD FTARYFAKRP 

       370        380        390        400        410        420 
LIGRNGSPII VVKGTILSTM AQFSLYMTLR YKLPLQILRL INIVYPWSHG DNYSDLSRKI 

       430        440        450        460        470        480 
KLAMRLVELY QPYLLFKGIF DDLNTERLRM KRKENIKELD GSFEFDPKSI DWDNYITNTH 

       490 
IPGLITHVLK Q 

References and documentation are available.

Number of amino acids: 491

Molecular weight: 55480.78

Theoretical pI: 8.36

Amino acid composition: 
Ala (A)  27	  5.5%
Arg (R)  23	  4.7%
Asn (N)  26	  5.3%
Asp (D)  30	  6.1%
Cys (C)   5	  1.0%
Gln (Q)  15	  3.1%
Glu (E)  26	  5.3%
Gly (G)  33	  6.7%
His (H)   9	  1.8%
Ile (I)  43	  8.8%
Leu (L)  61	 12.4%
Lys (K)  36	  7.3%
Met (M)  19	  3.9%
Phe (F)  20	  4.1%
Pro (P)  21	  4.3%
Ser (S)  25	  5.1%
Thr (T)  26	  5.3%
Trp (W)   5	  1.0%
Tyr (Y)  15	  3.1%
Val (V)  26	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2502
Hydrogen    H	      4008
Nitrogen    N	       660
Oxygen      O	       711
Sulfur      S	        24

Formula: C2502H4008N660O711S24
Total number of atoms: 7905

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50100
Abs 0.1% (=1 g/l)   0.903, assuming all pairs of Cys residues form cystines


Ext. coefficient    49850
Abs 0.1% (=1 g/l)   0.899, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.41
This classifies the protein as stable.



Aliphatic index: 103.46

Grand average of hydropathicity (GRAVY): -0.068