ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MESNLAGDSI PGPKWRKVAY GGMQIGYDDN YTDESFLEEM VMNANVVRRD LLKVMKDSVS 

        70         80         90        100        110        120 
ISQYLCIVAL VVLVWVHTLE SSLDENSLLL LDLSLLASGF LILLLTEEKM LSLSLLLRYL 

       130        140        150        160        170        180 
VNISFFTTGL YILAPIYQTL TRSISSDSIW AVTVSLLLLH LFLHDYSGST IRAPGALKTP 

       190        200        210        220        230        240 
NLTSCISVNA SIVASVFVAS RLPSRLHVFA VMLFSLQVFL FAPLVTYCIK KFNFGLHLLF 

       250        260        270        280        290        300 
SFALMGLTLY SIYALHRLFF LVFLSLVLLV NVVCPYWLIR MQEYKFEING PWDEAKLCFD 


ITD 

References and documentation are available.

Number of amino acids: 303

Molecular weight: 34200.44

Theoretical pI: 5.85

Amino acid composition: 
Ala (A)  17	  5.6%
Arg (R)  10	  3.3%
Asn (N)  11	  3.6%
Asp (D)  13	  4.3%
Cys (C)   5	  1.7%
Gln (Q)   5	  1.7%
Glu (E)  11	  3.6%
Gly (G)  12	  4.0%
His (H)   6	  2.0%
Ile (I)  18	  5.9%
Leu (L)  58	 19.1%
Lys (K)  10	  3.3%
Met (M)   9	  3.0%
Phe (F)  19	  6.3%
Pro (P)   9	  3.0%
Ser (S)  31	 10.2%
Thr (T)  14	  4.6%
Trp (W)   5	  1.7%
Tyr (Y)  13	  4.3%
Val (V)  27	  8.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 20

Atomic composition:

Carbon      C	      1595
Hydrogen    H	      2492
Nitrogen    N	       376
Oxygen      O	       426
Sulfur      S	        14

Formula: C1595H2492N376O426S14
Total number of atoms: 4903

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47120
Abs 0.1% (=1 g/l)   1.378, assuming all pairs of Cys residues form cystines


Ext. coefficient    46870
Abs 0.1% (=1 g/l)   1.370, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.55
This classifies the protein as stable.



Aliphatic index: 129.27

Grand average of hydropathicity (GRAVY): 0.692