ProtParam
User-provided sequence:
10 20 30 40 50 60
MESQQLSNYP HISHGSACAS VTSKEVHTNQ DPLDVSASKI QEYDKASTKA NSQQTTTPAS
70 80 90 100 110 120
SAVPENPHHA SPQPASVPPP QNGPYPQQCM MTQNQANPSG WSFYGHPSMI PYTPYQMSPM
130 140 150 160 170 180
YFPPGPQSQF PQYPSSVGTP LSTPSPESGN TFTDSSSADS DMTSTKKYVR PPPMLTSPND
190 200 210 220 230 240
FPNWVKTYIK FLQNSNLGGI IPTVNGKPVR PITDDELTFL YNAFQIFAPS QFLPTWVKDI
250 260 270 280 290 300
LSVDYTDIMK ILSKSIEKMQ SDTQEANDIV TLANLQYNGS TPADAFETKV TNIIDRLNNN
310 320 330 340 350 360
GIHINNKVAC QLIMRGLSGE YKFLRYTRHR HLNMTVAELF LDIHAIYEEQ QGSRNSKPNY
370 380 390 400 410 420
RRNPSDEKND SRSYTNTTKP KVIARNPQKT NNSKSKTARA HNVSTSNNSP STDNDSISKS
430 440
TTEPIQLNNK HDLHLRPETY
References and
documentation are available.
Number of amino acids: 440
Molecular weight: 49067.49
Theoretical pI: 7.73
Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 2145
Hydrogen H 3323
Nitrogen N 601
Oxygen O 691
Sulfur S 15
Formula: C
2145H
3323N
601O
691S
15
Total number of atoms: 6775
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44935
Abs 0.1% (=1 g/l) 0.916, assuming all pairs of Cys residues form cystines
Ext. coefficient 44810
Abs 0.1% (=1 g/l) 0.913, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.20
This classifies the protein as unstable.
Aliphatic index: 57.86
Grand average of hydropathicity (GRAVY): -0.815