ProtParam
User-provided sequence:
10 20 30 40 50 60
MESQQLSNYS PISHGSACAS VTSKEVHTNQ DPLDVSASKT EECEKASTKA NSQQTTTPAS
70 80 90 100 110 120
SAVPENPHHA SPQPASVPPP QNGPYPQQCM MTQNQANPSG WSFYGHPSMI PYTPYQMSPM
130 140 150 160 170 180
YFPPGPQSQF PQYPSSVGTP LSTPSPESGN TFTDSSSADS DMTSTKKYVR PPPMLTSPND
190 200 210 220 230 240
FPNWVKTYIK FLQNSNLGGI IPTVNGKPVR QITDDELTFL YNTFQIFAPS QFLPTWVKDI
250 260 270 280 290 300
LSVDYTDIMK ILSKSIEKMQ SDTQEANDIV TLANLQYNGS TPADAFETKV TNIIDRLNNN
310 320 330 340 350 360
GIHINNKVAC QLIMRGLSGE YKFLRYTRHR HLNMTVAELF LDIHAIYEEQ QGSRNSKPNY
370 380 390 400 410 420
RRNLSDEKND SRSYTNTTKP KVIARNPQKT NNSKSKTARA HNVSTSNNSP STDNDSISKS
430 440
TTEPIQLNNK HDLHLRPGTY
References and
documentation are available.
Number of amino acids: 440
Molecular weight: 48965.37
Theoretical pI: 7.70
Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 2134
Hydrogen H 3317
Nitrogen N 599
Oxygen O 693
Sulfur S 16
Formula: C
2134H
3317N
599O
693S
16
Total number of atoms: 6759
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 43570
Abs 0.1% (=1 g/l) 0.890, assuming all pairs of Cys residues form cystines
Ext. coefficient 43320
Abs 0.1% (=1 g/l) 0.885, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.64
This classifies the protein as unstable.
Aliphatic index: 57.64
Grand average of hydropathicity (GRAVY): -0.803