ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MESQQLSQHS PISHGSACAS VTSKEVHTNQ DPLDVSASKI QEYDKASTKA NSQQTTTPAS 

        70         80         90        100        110        120 
SAVPENPHHA SPQTAQSHSP QNGPYPQQCM MTQNQANPSD WSFYGRPSMI PYTPYQMSPM 

       130        140        150        160        170        180 
YFPPGPHSQF PQYPSSVGTP LSTPSPESGN TFTDSSSADS DMTSTKKYVR PPPMLTSPND 

       190        200        210        220        230        240 
FLNWVKTYIK FLQNSNLGGI IPTVNGKPVR QITDDELTFL YNTFQIFAPS QFLPTWVKDI 

       250        260        270        280        290        300 
LSVDYTDIMK ILSKSIEKMQ SDTQEANDIV TLANLQYNGS TPADAFETKV TNIIDRLNNN 

       310        320        330        340        350        360 
GIHINNKVAC QLIMRGLSGE YKFLRYTRHR HLNMTVAELF LDIHAIYEEQ QGSRNSKPNY 

       370        380        390        400        410        420 
RRNPSDEKND SRSYTNTTKP KVIARNPQKT NNSKSKTARA HNVSTSNNSP STDNDSISKS 

       430        440        450        460        470        480 
TTEPIQLNNK HDLHLGQKLT ESTVNHTNHS DDELPGHLLL DSGASRTLIR SAHHIHSASS 

       490        500        510        520        530        540 
NPDINVVDAQ KRNIPINAIG DLQFHFQDNT KTSIKVLHTP NIAYDLLSLN ELAAVDITAC 

       550        560        570        580        590        600 
FTKNVLERSD GTVLAPIVKY GDFYWVSKKY LLPSNISVPT INNVHTSEST RKYPYPFIHR 

       610        620        630        640        650        660 
MLAHANAQTI RYSLKNNTIT YFNESDVDWS SAIDYQCPDC LIGKSTKHRH IKGSRLKYQN 

       670        680        690        700        710        720 
SYEPFQYLHT DIFGPVHNLP KSAPSYFISF TDETTKFRWV YPLHDRREDS ILDVFTTILA 

       730        740        750        760        770        780 
FIKNQFQASV LVIQMDRGSE YTNRTLHKFL EKNGITPCYT TTADSRAHGV AERLNRTLLD 

       790        800        810        820        830        840 
DCRTQLQCSG LPNHLWFSAI EFSTIVRNSL ASPKSKKSAR QHAGLAGLDI STLLPFGQPV 

       850        860        870        880        890        900 
IVNDHNPNSK IHPRGIPGYA LHPSRNSYGY IIYLPSLKKT VDTTNYVILQ GKESRLDQFN 

       910        920        930        940        950        960 
YDALTFDEDL NRLTASYQSF IASNEIQQSD DLNFQSDIEL HPEQPRNVLS KAVSPTDSTP 

       970        980        990       1000       1010       1020 
PSTHTEDSKR VSKTNIRAPR EVDPNISKSD ILPSKKRSST PQISDIESTG SGGMHRLDVP 

      1030       1040       1050       1060       1070       1080 
LLAPMSQSNT HESSHTSKSK DFRHSDSYSD NETNHTNVPI SSTGGTNNKT VPQTSEQETE 

      1090       1100       1110       1120       1130       1140 
KRIIHRSPSI DTSSSESNSL HHVVPIKTSD TCPKENTEES IIADLPLPDL PPEPPTKLSD 

      1150       1160       1170       1180       1190       1200 
SFKELPPINS RQTNSSLGGI GDSNAYTTIN SKKRSLEDNE TEIKVSRDTW NTKNMRSLEP 

      1210       1220       1230       1240       1250       1260 
PRSKKRIHLI AAVKAVKSIK PIRTTLRYDE AITYNKDIKE KEKYIEAYHK EVNQLLKMKT 

      1270       1280       1290       1300       1310       1320 
WDTDKYYDRK EIDPKRVINS MFIFNRKRDG THKARFVARG DIQHPDTYDS GMQSNTVHHY 

      1330       1340       1350       1360       1370       1380 
ALMTSLSLAL DNNYYITQLD ISSAYLYADI KEELYIRPPP HLGMNDKLIR LKKSLYGLKQ 

      1390       1400       1410       1420       1430       1440 
SGANWYETIK SYLIEQCDME EVRGWSCVFK NSQVTICLFV DDMILFSKDL NANKKIITTL 

      1450       1460       1470       1480       1490       1500 
KKQYDTKIIN LGESDNEIQY DILGLEIKYQ RSKYMKLGME KSLTEKLPKL NVHLNPKGKK 

      1510       1520       1530       1540       1550       1560 
LSAPGQPGLY IDQDELEIDE DEYKEKVHEM QKLIGLASYV GYKFRFDLLY YINTLAQHIL 

      1570       1580       1590       1600       1610       1620 
FPSRQVLDMT YELIQFMWDT RDKQLIWHKN KPTKPDNKLV AISDASYGNQ PYYKSQIGNI 

      1630       1640       1650       1660       1670       1680 
FLLNGKVIGG KSTKASLTCT STTEAEIHAV SEAIPLLNNL SHLVQELNKK PIIKGLLTDS 

      1690       1700       1710       1720       1730       1740 
RSTISIIKST NEEKFRNRFF GTKAMRLRDE VSGNNLYVYY IETKKNIADV MTKPLPIKTF 


KLLTNKWIH 

References and documentation are available.

Number of amino acids: 1749

Molecular weight: 198198.19

Theoretical pI: 8.68

Amino acid composition: 
Ala (A)  80	  4.6%
Arg (R)  73	  4.2%
Asn (N) 120	  6.9%
Asp (D) 108	  6.2%
Cys (C)  14	  0.8%
Gln (Q)  76	  4.3%
Glu (E)  83	  4.7%
Gly (G)  65	  3.7%
His (H)  61	  3.5%
Ile (I) 125	  7.1%
Leu (L) 144	  8.2%
Lys (K) 133	  7.6%
Met (M)  31	  1.8%
Phe (F)  52	  3.0%
Pro (P) 106	  6.1%
Ser (S) 181	 10.3%
Thr (T) 137	  7.8%
Trp (W)  14	  0.8%
Tyr (Y)  75	  4.3%
Val (V)  71	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 191
Total number of positively charged residues (Arg + Lys): 206

Atomic composition:

Carbon      C	      8763
Hydrogen    H	     13781
Nitrogen    N	      2433
Oxygen      O	      2721
Sulfur      S	        45

Formula: C8763H13781N2433O2721S45
Total number of atoms: 27743

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   189625
Abs 0.1% (=1 g/l)   0.957, assuming all pairs of Cys residues form cystines


Ext. coefficient   188750
Abs 0.1% (=1 g/l)   0.952, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.40
This classifies the protein as unstable.



Aliphatic index: 76.33

Grand average of hydropathicity (GRAVY): -0.659