ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MESQQLSQHS PISHGSACAS VTSKEVQTTQ DPLDISASKT EECEKVFTQA NSQQPTTPPS 

        70         80         90        100        110        120 
AAVPENHHHA SPQAAQVPLP QNGPYPQQRM MTPQQANISG WPVYGHPSLM PYPPYQMSPM 

       130        140        150        160        170        180 
YAPPGAQSQF TQYPQYVGTH LNTPSPESGN SFPDSSSAKS NMTSTNQHVR PPPILTSPND 

       190        200        210        220        230        240 
FLNWVKIYIK FLQNSNLGDI IPTATRKAVR QMTDDELTFL CHTFQLFALS QFLPTWVKDI 

       250        260        270        280        290        300 
LSVDYTDIMK ILSKSINKMQ SDTQEVNDIT TLANLHYNGS TPADAFEAEV TNILDRLKNN 

       310        320        330        340        350        360 
GIPINNKVAC QFIMRGLSGE YKFLRYARHR YIHMTVADLF SDIHSMYEEQ QESKRNKSTY 

       370        380        390        400        410        420 
RRSPSDEKKD SRTYTNTTKP KSITRNSQKP NNSQSRTARA HNVSTSNNFP GPDNDLIRGS 

       430        440 
TTEPIQLKNK HDLHLRPGTY 

References and documentation are available.

Number of amino acids: 440

Molecular weight: 49445.20

Theoretical pI: 8.48

Amino acid composition: 
Ala (A)  25	  5.7%
Arg (R)  18	  4.1%
Asn (N)  31	  7.0%
Asp (D)  21	  4.8%
Cys (C)   4	  0.9%
Gln (Q)  33	  7.5%
Glu (E)  17	  3.9%
Gly (G)  15	  3.4%
His (H)  16	  3.6%
Ile (I)  22	  5.0%
Leu (L)  27	  6.1%
Lys (K)  23	  5.2%
Met (M)  13	  3.0%
Phe (F)  14	  3.2%
Pro (P)  39	  8.9%
Ser (S)  47	 10.7%
Thr (T)  38	  8.6%
Trp (W)   3	  0.7%
Tyr (Y)  17	  3.9%
Val (V)  17	  3.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      2161
Hydrogen    H	      3362
Nitrogen    N	       616
Oxygen      O	       683
Sulfur      S	        17

Formula: C2161H3362N616O683S17
Total number of atoms: 6839

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42080
Abs 0.1% (=1 g/l)   0.851, assuming all pairs of Cys residues form cystines


Ext. coefficient    41830
Abs 0.1% (=1 g/l)   0.846, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.51
This classifies the protein as unstable.



Aliphatic index: 60.32

Grand average of hydropathicity (GRAVY): -0.783