ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MESQQLSQNS PNLHGSAYAS VTSKEVPSNQ DPLAVSASNL PEFDRDSTKV NSQQETTPGT 

        70         80         90        100        110        120 
SAVPENHHHV SPQPASVPPP QNGQYQQHGM MTPNKAMASN WAHYQQPSMM TCSHYQTSPA 

       130        140        150        160        170        180 
YYQPDPHYPL PQYIPPLSTS SPDPIDLKNQ HSEIPQAKTK VGNNVLPPHT LTSEENFSTW 

       190        200        210        220        230        240 
VKFYIRFLKN SNLGDIIPND QGEIKRQMTY EEHAYIYNTF QAFAPFHLLP TWVKQILEIN 

       250        260        270        280        290        300 
YADILTVLCK SVSKMQTNNQ ELKDWIALAN LEYDGSTSAD TFEITVSTII QRLKENNINV 

       310        320        330        340        350        360 
SDRLACQLIL KGLSGDFKYL RNQYRTKTNM KLSQLFAEIQ LIYDENKIMN LNKPSQYKQH 

       370        380        390        400        410        420 
SEYKNVSRTS PNTTNTKVTT RNYQRTNSSK PRAAKAHNIA TSSKFSRVNN DHINESTVSS 

       430        440        450        460        470        480 
QYLSDDNELS LGQQQKESKP THTIDSNDEL PDHLLIDSGA SQTLVRSAHY LHHATPNSEI 

       490        500        510        520        530        540 
NIVDAQKQDI PINAIGNLHF NFQNGTKTSI KALHTPNIAY DLLSLSELAN QNITACFTRN 

       550        560        570        580        590        600 
TLERSDGTVL APIVKHGDFY WLSKKYLIPS HISKLTINNV NKSKSVNKYP YPLIHRMLGH 

       610        620        630        640        650        660 
ANFRSIQKSL KKNAVTYLKE SDIEWSNAST YQCPDCLIGK STKHRHVKGS RLKYQESYEP 

       670        680        690        700        710        720 
FQYLHTDIFG PVHHLPKSAP SYFISFTDEK TRFQWVYPLH DRREESILNV FTSILAFIKN 

       730        740        750        760        770        780 
QFNARVLVIQ MDRGSEYTNK TLHKFFTNRG ITACYTTTAD SRAHGVAERL NRTLLNDCRT 

       790        800        810        820        830        840 
LLHCSGLPNH LWFSAVEFST IIRNSLVSPK NDKSARQHAG LAGLDITTIL PFGQPVIVNN 

       850        860        870        880        890        900 
HNPDSKIHPR GIPGYALHPS RNSYGYIIYL PSLKKTVDTT NYVILQDKQS KLDQFNYDTL 

       910        920        930        940        950        960 
TFDDDLNRLT AHNQSFIEKN ETEQSYDQNT ESDHDYQSEI EINSDPLVND FSSQSINPLQ 

       970        980        990       1000       1010       1020 
LDKEPVQKVR APKEVDADIS EYNILPSTIR SRTPHIINKE STEMGGTIES DTTSPRHSST 

      1030       1040       1050       1060       1070       1080 
FTARNQKRPG SPNDMIDLTS QDRVNYGLEN IKTTRLGGTE EPYIQRNSDT NIKYRTTNST 

      1090       1100       1110       1120       1130       1140 
PSIDDRSSNS ESTTPIISIE TKAACDNTPS IDTDPPEYRS SDHATPNIMP DKSSKNVTAD 

      1150       1160       1170       1180       1190       1200 
SILDDLPLPD LTNKSPTDTS DVSKDIPHIH SRQTNSSLGG MDDSNVLTTT KSKKRSLEDN 

      1210       1220       1230       1240       1250       1260 
ETEIEVSRDT WNNKNMRSLE PPRSKKRINL IAAIKGVKSI KPVRTTLRYD EAITYNEDNK 

      1270       1280       1290       1300       1310       1320 
EKDRYIEAYH KEINQLLRMN TWDTNKYYDR NDIDPKKVIN SMFIFNKKRD GTHKARFVAR 

      1330       1340       1350       1360       1370       1380 
GDIQHPDTYD SDMQSNTVHH YALMTSLSIA LDNDYYITQL DISSAYLYAD IKEELYIRPP 

      1390       1400       1410       1420       1430       1440 
PHLGLNDKLL RLRKSLYGLK QSGANWYETI KSYLINCCDM QEVRGWSCVF KNSQVTICLF 

      1450       1460       1470       1480       1490       1500 
VDDMILFSKD LNANKKIITT LKKQYDTKII NLGEGDNEIQ YDILGLEIKY QRSKYMKLGM 

      1510       1520       1530       1540       1550       1560 
EKSLTEKLPK LNVPLNPKGK KLRAPGQPGH YIDQDELEID EDEYKEKVHE MQKLIGLASY 

      1570       1580       1590       1600       1610       1620 
VGYKFRFDLL YYINTLAQHI LFPSRQVLDM TYELIQFMWD TRDKQLIWHK NKPTKPDNKL 

      1630       1640       1650       1660       1670       1680 
VAISDASYGN QPYYKSQIGN IFLLNGKVIG GKSTKASLTC TSTTEAEIHA VSEAIPLLNN 

      1690       1700       1710       1720       1730       1740 
LSHLVQELNK KPIIKGLLTD SRSTISIIKS TNEEKFRNRF FGTKAMRLRD EVSGNNLYVY 

      1750       1760       1770 
YIETKKNIAD VMTKPLPIKT FKLLTNKWIH 

References and documentation are available.

Number of amino acids: 1770

Molecular weight: 201965.23

Theoretical pI: 8.34

Amino acid composition: 
Ala (A)  80	  4.5%
Arg (R)  74	  4.2%
Asn (N) 136	  7.7%
Asp (D) 113	  6.4%
Cys (C)  15	  0.8%
Gln (Q)  84	  4.7%
Glu (E)  88	  5.0%
Gly (G)  62	  3.5%
His (H)  60	  3.4%
Ile (I) 130	  7.3%
Leu (L) 152	  8.6%
Lys (K) 135	  7.6%
Met (M)  30	  1.7%
Phe (F)  49	  2.8%
Pro (P)  97	  5.5%
Ser (S) 163	  9.2%
Thr (T) 137	  7.7%
Trp (W)  15	  0.8%
Tyr (Y)  82	  4.6%
Val (V)  68	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 201
Total number of positively charged residues (Arg + Lys): 209

Atomic composition:

Carbon      C	      8927
Hydrogen    H	     14026
Nitrogen    N	      2482
Oxygen      O	      2775
Sulfur      S	        45

Formula: C8927H14026N2482O2775S45
Total number of atoms: 28255

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   205555
Abs 0.1% (=1 g/l)   1.018, assuming all pairs of Cys residues form cystines


Ext. coefficient   204680
Abs 0.1% (=1 g/l)   1.013, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.17
This classifies the protein as unstable.



Aliphatic index: 77.80

Grand average of hydropathicity (GRAVY): -0.694