ProtParam
User-provided sequence:
10 20 30 40 50 60
MESQQLSQNS PTFHGSAYAS VTSKEVPSNQ DPLAVSASNL PEFDRDSTKV NSQQETTPGT
70 80 90 100 110 120
SAVPENHHHV SPQPASVPPP QNGQYQQHGM MTPNKAMASN WAHYQQPSMM TCSHYQTSPA
130 140 150 160 170 180
YYQPDPHYPL PQYIPPLSTS SPDPIGSQDQ HSEVPQAKTK VRNNVLPPHT LTSEENFSTW
190 200 210 220 230 240
VKFYIRFLKN SNLGDIIPND QGEIKRQMTY EEHAYIYNTF QAFAPFHLLP TWVKQILEIN
250 260 270 280 290 300
YSDILTVLCK SVSKMQTNNQ ELKDWIALAN LEYNGSTSAD TFEITVSTII QRLKENNINV
310 320 330 340 350 360
SDRLACQLIL KGLSGDFKYL RNQYRTKTNM KLSQLFAEIQ LIYDENKIMN LNKPSQYKQH
370 380 390 400 410 420
SEYKNVSRTS PNTTNTKVTT RNYHRTNSSK PRAAKAHNIA TSSKFSRVNN DHINESTVSS
430
QYLSDDNELS LRPATERI
References and
documentation are available.
Number of amino acids: 438
Molecular weight: 49736.35
Theoretical pI: 7.76
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 2182
Hydrogen H 3383
Nitrogen N 615
Oxygen O 693
Sulfur S 13
Formula: C
2182H
3383N
615O
693S
13
Total number of atoms: 6886
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 53415
Abs 0.1% (=1 g/l) 1.074, assuming all pairs of Cys residues form cystines
Ext. coefficient 53290
Abs 0.1% (=1 g/l) 1.071, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.12
This classifies the protein as unstable.
Aliphatic index: 65.02
Grand average of hydropathicity (GRAVY): -0.801