ProtParam
User-provided sequence:
10 20 30 40 50 60
MESSQGRRRR PGTVVPGEAA ETDSELSASS SEEELYLGPS GPTRGRPTGL RVAGEAAETD
70 80 90 100 110 120
SEPEPEPTVV PVDLPPLVVQ RDPAETWGTE ETPAMAPARS LLQLRLAESQ TRLDHDVAAA
130 140 150 160 170 180
VSGVYRRAGR DVAALAGRLA AAQATGLAAA HSVRLARGDL CALAERLDIV AGCRLLPDIR
190
GVPGMEPEQD PGPRA
References and
documentation are available.
Number of amino acids: 195
Molecular weight: 20397.79
Theoretical pI: 4.84
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 21
Atomic composition:
Carbon C 868
Hydrogen H 1425
Nitrogen N 269
Oxygen O 288
Sulfur S 5
Formula: C
868H
1425N
269O
288S
5
Total number of atoms: 2855
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 8605
Abs 0.1% (=1 g/l) 0.422, assuming all pairs of Cys residues form cystines
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 0.416, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 59.72
This classifies the protein as unstable.
Aliphatic index: 81.69
Grand average of hydropathicity (GRAVY): -0.376