ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MESTAIVPKG PVVGSEDMEK AEVIASGSTD IISTTSTATT TAAIGSVQEE SVKQEDVPME 

        70         80         90        100        110        120 
GGEGEVEEEE GETRCICGEL DTPDDSGFFI QCEQCSSWQH GYCVSITQDN APDKYWCEQC 

       130        140        150        160        170        180 
RPELHQLFTT DTGEARSIYK PVQEKRRQSR RKARSAAASK SHAANEAEKS PRNTSNTDDN 

       190        200        210        220        230        240 
VDDIGDEEDE VEDEASAVAL AKDGNTRSSR RRRRNSMDDA STDQYSLDPG DSDKKLLDRK 

       250        260        270        280        290        300 
RATFMAREEK QYQRMLEKAL KESRRTSHQE DPESYENDAD IYQGDTDNHN GTTRLQTDVM 

       310        320        330        340        350        360 
LTEGKPDSVT NDDMKESLRP SKEQSMEKTN DVEKEASQEK ESSTGSAQDT EKTDEPILPL 

       370        380        390        400        410        420 
TSISSSEDDS RKASSRGSKR VSKPARKGNR TRRSNTSSDT NQNRRSADIG TDKPVKPRLP 

       430        440        450        460        470        480 
PQRTSLNEMR RRVSAILEFI SRTQWELSED QSDREEFVRF VENQHFVEKV DTIYNGYNES 

       490        500 
LSMMDDLTRE LLLWEKKYSN NTNAIQ 

References and documentation are available.

Number of amino acids: 506

Molecular weight: 57052.92

Theoretical pI: 4.81

Amino acid composition: 
Ala (A)  31	  6.1%
Arg (R)  41	  8.1%
Asn (N)  23	  4.5%
Asp (D)  46	  9.1%
Cys (C)   7	  1.4%
Gln (Q)  25	  4.9%
Glu (E)  57	 11.3%
Gly (G)  23	  4.5%
His (H)   6	  1.2%
Ile (I)  18	  3.6%
Leu (L)  23	  4.5%
Lys (K)  32	  6.3%
Met (M)  12	  2.4%
Phe (F)   8	  1.6%
Pro (P)  19	  3.8%
Ser (S)  58	 11.5%
Thr (T)  40	  7.9%
Trp (W)   4	  0.8%
Tyr (Y)  10	  2.0%
Val (V)  23	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 103
Total number of positively charged residues (Arg + Lys): 73

Atomic composition:

Carbon      C	      2376
Hydrogen    H	      3817
Nitrogen    N	       725
Oxygen      O	       869
Sulfur      S	        19

Formula: C2376H3817N725O869S19
Total number of atoms: 7806

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37275
Abs 0.1% (=1 g/l)   0.653, assuming all pairs of Cys residues form cystines


Ext. coefficient    36900
Abs 0.1% (=1 g/l)   0.647, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.12
This classifies the protein as unstable.



Aliphatic index: 50.91

Grand average of hydropathicity (GRAVY): -1.194