ProtParam

User-provided sequence:

        10         20         30         40         50         60 
METILQPKAR PFESLKRKRF REWLRPSTAH GSLLHSDTLD LRDFAKPNPA DTFSNLDSGH 

        70         80         90        100        110        120 
CPLVTTPIKY ECPDGKSSFF RGDTKFETLF SNRKFYEFKD NLKRGLKKIR HGRNGHQSEK 

       130        140        150        160        170        180 
RCPVVEETKK SVSDNLDKPD NNTPCFDRFH TNSKEFETQF DHSNRSQNSE KAYLDNESCW 

       190        200        210        220        230        240 
NLSEKFIPFN NLKYEDLKHF EENLQSLAPA TFTPIESNES LDRSDSTRGT KRSIRNDSSD 

       250        260        270        280        290        300 
TTSEKRLCLK QYSDEPESDH SMESTPSIYI TKEVQERIEA LSSTDSFLIE KVDFPSNKIG 

       310        320        330        340        350        360 
SSASDYESDN EYRNMDEDSI NDVTTEKEGN VVIPDSNTST VDAMEKPIEV SSALKDDTLD 

       370        380        390        400        410        420 
KDIDDASSSY SDDVETTFEP VESEELSDLS DTSSSGSSKI YTIPTFRGLT NRTNISQILS 

       430        440        450        460        470        480 
KVGKADLSQD NLTHLIKSHQ KKKRCVNFRN KRFYDAFNPY VDNEEDAELS DSENISEMDT 

       490        500        510        520        530        540 
DLCIKDRSTS SVRFDENSRL LIYKKSKKLN KDETQSGYST TEMRSILKTK MNSQHDEESQ 

       550        560        570 
RASKCDTVGV AQFLHYFQYT EYKRQRNEAE IID 

References and documentation are available.

Number of amino acids: 573

Molecular weight: 65837.23

Theoretical pI: 5.15

Amino acid composition: 
Ala (A)  18	  3.1%
Arg (R)  33	  5.8%
Asn (N)  36	  6.3%
Asp (D)  54	  9.4%
Cys (C)   9	  1.6%
Gln (Q)  16	  2.8%
Glu (E)  52	  9.1%
Gly (G)  15	  2.6%
His (H)  13	  2.3%
Ile (I)  26	  4.5%
Leu (L)  40	  7.0%
Lys (K)  49	  8.6%
Met (M)   7	  1.2%
Phe (F)  28	  4.9%
Pro (P)  22	  3.8%
Ser (S)  75	 13.1%
Thr (T)  42	  7.3%
Trp (W)   2	  0.3%
Tyr (Y)  17	  3.0%
Val (V)  19	  3.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 106
Total number of positively charged residues (Arg + Lys): 82

Atomic composition:

Carbon      C	      2837
Hydrogen    H	      4443
Nitrogen    N	       801
Oxygen      O	       972
Sulfur      S	        16

Formula: C2837H4443N801O972S16
Total number of atoms: 9069

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36830
Abs 0.1% (=1 g/l)   0.559, assuming all pairs of Cys residues form cystines


Ext. coefficient    36330
Abs 0.1% (=1 g/l)   0.552, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.43
This classifies the protein as unstable.



Aliphatic index: 57.68

Grand average of hydropathicity (GRAVY): -1.035