ProtParam
User-provided sequence:
10 20 30 40 50 60
METNFSFDSN LIVIIIITLF ATRIIAKRFL STPKMVSQET VAHVKDLIGQ KEVFVAAKTY
70 80 90 100 110 120
CPYCKATLST LFQELNVPKS KALVLELDEM SNGSEIQDAL EEISGQKTVP NVYINGKHIG
130 140
GNSDLETLKK NGKLAEILKP VFQ
References and
documentation are available.
Number of amino acids: 143
Molecular weight: 15861.47
Theoretical pI: 6.73
Total number of negatively charged residues (Asp + Glu): 16
Total number of positively charged residues (Arg + Lys): 16
Atomic composition:
Carbon C 715
Hydrogen H 1161
Nitrogen N 181
Oxygen O 213
Sulfur S 5
Formula: C
715H
1161N
181O
213S
5
Total number of atoms: 2275
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 4595
Abs 0.1% (=1 g/l) 0.290, assuming all pairs of Cys residues form cystines
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.282, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.24
This classifies the protein as stable.
Aliphatic index: 104.97
Grand average of hydropathicity (GRAVY): 0.024