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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
METNFSFDSN LIVIIIITLF ATRIIAKRFL STPKMVSQET VAHVKDLIGQ KEVFVAAKTY

70 80 90 100 110 120
CPYCKATLST LFQELNVPKS KALVLELDEM SNGSEIQDAL EEISGQKTVP NVYINGKHIG

130 140
GNSDLETLKK NGKLAEILKP VFQ


References and documentation are available.

Number of amino acids: 143 Molecular weight: 15861.47 Theoretical pI: 6.73
Amino acid composition: 
Ala (A) 9 6.3% Arg (R) 2 1.4% Asn (N) 8 5.6% Asp (D) 5 3.5% Cys (C) 2 1.4% Gln (Q) 6 4.2% Glu (E) 11 7.7% Gly (G) 7 4.9% His (H) 2 1.4% Ile (I) 13 9.1% Leu (L) 15 10.5% Lys (K) 14 9.8% Met (M) 3 2.1% Phe (F) 7 4.9% Pro (P) 5 3.5% Ser (S) 10 7.0% Thr (T) 10 7.0% Trp (W) 0 0.0% Tyr (Y) 3 2.1% Val (V) 11 7.7% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 16 Total number of positively charged residues (Arg + Lys): 16 Atomic composition: Carbon C 715 Hydrogen H 1161 Nitrogen N 181 Oxygen O 213 Sulfur S 5 Formula: C715H1161N181O213S5 Total number of atoms: 2275 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 4595 Abs 0.1% (=1 g/l) 0.290, assuming all pairs of Cys residues form cystines Ext. coefficient 4470 Abs 0.1% (=1 g/l) 0.282, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 32.24 This classifies the protein as stable. Aliphatic index: 104.97 Grand average of hydropathicity (GRAVY): 0.024