ProtParam

User-provided sequence:

        10         20         30         40         50         60 
METPLDLLKL NLDERVYIKL RGARTLVGTL QAFDSHCNIV LSDAVETIYQ LNNEELSESE 

        70         80 
RRCEMVFIRG DTVTLISTPS EDDDGAVEI 

References and documentation are available.

Number of amino acids: 89

Molecular weight: 10030.30

Theoretical pI: 4.29

Amino acid composition: 
Ala (A)   4	  4.5%
Arg (R)   6	  6.7%
Asn (N)   4	  4.5%
Asp (D)   8	  9.0%
Cys (C)   2	  2.2%
Gln (Q)   2	  2.2%
Glu (E)  10	 11.2%
Gly (G)   4	  4.5%
His (H)   1	  1.1%
Ile (I)   6	  6.7%
Leu (L)  12	 13.5%
Lys (K)   2	  2.2%
Met (M)   2	  2.2%
Phe (F)   2	  2.2%
Pro (P)   2	  2.2%
Ser (S)   6	  6.7%
Thr (T)   7	  7.9%
Trp (W)   0	  0.0%
Tyr (Y)   2	  2.2%
Val (V)   7	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 8

Atomic composition:

Carbon      C	       433
Hydrogen    H	       705
Nitrogen    N	       117
Oxygen      O	       147
Sulfur      S	         4

Formula: C433H705N117O147S4
Total number of atoms: 1406

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     3105
Abs 0.1% (=1 g/l)   0.310, assuming all pairs of Cys residues form cystines


Ext. coefficient     2980
Abs 0.1% (=1 g/l)   0.297, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.47
This classifies the protein as stable.



Aliphatic index: 106.18

Grand average of hydropathicity (GRAVY): -0.174