ProtParam

User-provided sequence:

        10         20         30         40         50         60 
METQSTGTED GFTPVTHRGG RRAKKRQAEQ SSAAGQDGEA GRMDTEEARP AKRPVFPPLS 

        70         80         90        100        110        120 
GDQLLTGKEE TRKIPVPGNR YTPLKENWMK IFTPIVEHLG LQIRFNLKSR NVEIRTCKDT 

       130        140        150        160        170        180 
KDVSALTKAA DFVKAFVLGF QVEDALALIR LDDLFLESFE ITDVKPLKGD HLSRAIGRIA 

       190        200        210        220        230        240 
GKGGKTKFTI ENVTRTRIVL ADVHVHILGS FQNIKMARTA ICNLILGNPP SKVYGNIRAV 


ASRSADRF 

References and documentation are available.

Number of amino acids: 248

Molecular weight: 27453.54

Theoretical pI: 9.80

Amino acid composition: 
Ala (A)  21	  8.5%
Arg (R)  21	  8.5%
Asn (N)   9	  3.6%
Asp (D)  14	  5.6%
Cys (C)   2	  0.8%
Gln (Q)   8	  3.2%
Glu (E)  15	  6.0%
Gly (G)  20	  8.1%
His (H)   5	  2.0%
Ile (I)  16	  6.5%
Leu (L)  20	  8.1%
Lys (K)  19	  7.7%
Met (M)   4	  1.6%
Phe (F)  12	  4.8%
Pro (P)  12	  4.8%
Ser (S)  12	  4.8%
Thr (T)  19	  7.7%
Trp (W)   1	  0.4%
Tyr (Y)   2	  0.8%
Val (V)  16	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1211
Hydrogen    H	      1974
Nitrogen    N	       358
Oxygen      O	       357
Sulfur      S	         6

Formula: C1211H1974N358O357S6
Total number of atoms: 3906

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     8605
Abs 0.1% (=1 g/l)   0.313, assuming all pairs of Cys residues form cystines


Ext. coefficient     8480
Abs 0.1% (=1 g/l)   0.309, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.06
This classifies the protein as unstable.



Aliphatic index: 83.79

Grand average of hydropathicity (GRAVY): -0.403