ProtParam

User-provided sequence:

        10         20         30         40         50         60 
METSSFENAP PAAINDAQDN NINTETNDQE TNQQSIETRD AIDKENGVQT ETGENSAKNA 

        70         80         90        100        110        120 
EQNVSSTNLN NAPTNGALDD DVIPNAIVIK NIPFAIKKEQ LLDIIEEMDL PLPYAFNYHF 

       130        140        150        160        170        180 
DNGIFRGLAF ANFTTPEETT QVITSLNGKE ISGRKLKVEY KKMLPQAERE RIEREKREKR 

       190        200        210        220        230        240 
GQLEEQHRSS SNLSLDSLSK MSGSGNNNTS NNQLFSTLMN GINANSMMNS PMNNTINNNS 

       250        260        270        280        290        300 
SNNNNSGNII LNQPSLSAQH TSSSLYQTNV NNQAQMSTER FYAPLPSTST LPLPPQQLDF 

       310        320        330        340        350        360 
NDPDTLEIYS QLLLFKDREK YYYELAYPMG ISASHKRIIN VLCSYLGLVE VYDPRFIIIR 

       370        380        390        400        410        420 
RKILDHANLQ SHLQQQGQMT SAHPLQPNST GGSMNRSQSY TSLLQAHAAA AANSISNQAV 

       430        440        450        460        470        480 
NNSSNSNTIN SNNGNGNNVI INNNSASSTP KISSQGQFSM QPTLTSPKMN IHHSSQYNSA 

       490        500        510        520        530        540 
DQPQQPQPQT QQNVQSAAQQ QQSFLRQQAT LTPSSRIPSG YSANHYQINS VNPLLRNSQI 

       550        560        570        580        590        600 
SPPNSQIPIN SQTLSQAQPP AQSQTQQRVP VAYQNASLSS QQLYNLNGPS SANSQSQLLP 

       610        620        630        640        650        660 
QHTNGSVHSN FSYQSYHDES MLSAHNLNSA DLIYKSLSHS GLDDGLEQGL NRSLSGLDLQ 


NQNKKNLW 

References and documentation are available.

Number of amino acids: 668

Molecular weight: 73775.82

Theoretical pI: 5.82

Amino acid composition: 
Ala (A)  43	  6.4%
Arg (R)  21	  3.1%
Asn (N)  86	 12.9%
Asp (D)  24	  3.6%
Cys (C)   1	  0.1%
Gln (Q)  67	 10.0%
Glu (E)  30	  4.5%
Gly (G)  27	  4.0%
His (H)  16	  2.4%
Ile (I)  39	  5.8%
Leu (L)  59	  8.8%
Lys (K)  22	  3.3%
Met (M)  15	  2.2%
Phe (F)  15	  2.2%
Pro (P)  38	  5.7%
Ser (S)  90	 13.5%
Thr (T)  36	  5.4%
Trp (W)   1	  0.1%
Tyr (Y)  21	  3.1%
Val (V)  17	  2.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      3152
Hydrogen    H	      4981
Nitrogen    N	       939
Oxygen      O	      1077
Sulfur      S	        16

Formula: C3152H4981N939O1077S16
Total number of atoms: 10165

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36790
Abs 0.1% (=1 g/l)   0.499, assuming all pairs of Cys residues form cystines


Ext. coefficient    36790
Abs 0.1% (=1 g/l)   0.499, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.78
This classifies the protein as unstable.



Aliphatic index: 71.03

Grand average of hydropathicity (GRAVY): -0.796