ProtParam
User-provided sequence:
10 20 30 40 50 60
METSSNLSSM VRLNIGGKKF CTTIDTLTIR EPDSMLAAMF SGRHAMCQES KKGYVFIDRD
70 80 90 100 110 120
GKHFRHILNW LRDGVIPSLS DPDCSELLRE ADYYQLLGLK DGIKDSRKEV GEVEAELTRI
130 140 150 160 170 180
DIIKCIQTER VRFRGVNLSG IDLSKLDLSL VDFSYACLRN VFFSRTNLQC AKFRNADAEG
190 200 210 220 230 240
SIFHNAILRE CEFTSANLRG ALLAGTNLQS ANLQDACLVG CSFCGADLRT AHLQNADLTN
250 260 270 280 290
ANLEGANLEG ANLKGAKLSN ANFKGANLQR AYLRHVNLRE AVSPKPILGY
References and
documentation are available.
Number of amino acids: 290
Molecular weight: 32101.63
Theoretical pI: 8.01
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1398
Hydrogen H 2251
Nitrogen N 409
Oxygen O 427
Sulfur S 15
Formula: C
1398H
2251N
409O
427S
15
Total number of atoms: 4500
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15065
Abs 0.1% (=1 g/l) 0.469, assuming all pairs of Cys residues form cystines
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.450, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.74
This classifies the protein as stable.
Aliphatic index: 92.24
Grand average of hydropathicity (GRAVY): -0.208