ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEVAPALSTT QSDVAFQKVE THEIDRSSYL GPCYNSDELM QLISAYYNVE PLVGYLEQHP 

        70         80         90        100        110        120 
EYQNVTLQFP DDLIKDSSLI VRLLQSKFPH GKIKFWVLAD TAYSACCVDE VAAEHVHAEV 

       130        140        150        160        170        180 
VVHFGDACLN AIQNLPVVYS FGTPFLDLAL VVENFQRAFP DLSSKICLMA NAPFSKHLSQ 

       190        200        210        220        230        240 
LYNILKGDLH YTNIIYSQVN TSAVEEKFVT ILDTFHVPED VDQVGVFEKN SVLFGQHDKA 

       250        260        270        280        290        300 
DNISPEDYHL FHLTTPQDPR LLYLSTVFQS VHIFDPALPG MVTGPFPSLM RRYKYMHVAR 

       310        320        330        340        350        360 
TAGCIGILVN TLSLRNTRET INELVKLIKT REKKHYLFVV GKPNVAKLAN FEDIDIWCIL 

       370        380        390        400        410        420 
GCSQSGIIVD QFNEFYKPII TPYELNLALS EEVTWTGKWV VDFRDAIDEI EQNLGGQDTI 

       430        440        450        460        470        480 
SASTTSDEPE FDVVRGRYTS TSRPLRALTH LELEAADDDD SKQLTTRHTA SGAVIKGTVS 

       490        500        510        520        530 
TSASALQNRS WKGLGSDFDS TEVDNTGADI EEGISGVARG YGFDREDAMK KENK 

References and documentation are available.

Number of amino acids: 534

Molecular weight: 59775.43

Theoretical pI: 4.93

Amino acid composition: 
Ala (A)  36	  6.7%
Arg (R)  19	  3.6%
Asn (N)  23	  4.3%
Asp (D)  39	  7.3%
Cys (C)   8	  1.5%
Gln (Q)  21	  3.9%
Glu (E)  35	  6.6%
Gly (G)  28	  5.2%
His (H)  17	  3.2%
Ile (I)  30	  5.6%
Leu (L)  51	  9.6%
Lys (K)  26	  4.9%
Met (M)   7	  1.3%
Phe (F)  26	  4.9%
Pro (P)  23	  4.3%
Ser (S)  40	  7.5%
Thr (T)  35	  6.6%
Trp (W)   5	  0.9%
Tyr (Y)  20	  3.7%
Val (V)  45	  8.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 74
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      2678
Hydrogen    H	      4140
Nitrogen    N	       700
Oxygen      O	       822
Sulfur      S	        15

Formula: C2678H4140N700O822S15
Total number of atoms: 8355

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57800
Abs 0.1% (=1 g/l)   0.967, assuming all pairs of Cys residues form cystines


Ext. coefficient    57300
Abs 0.1% (=1 g/l)   0.959, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.13
This classifies the protein as stable.



Aliphatic index: 90.34

Grand average of hydropathicity (GRAVY): -0.188