ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEVITVREAL RLAMEEEMER DQSVFLMGEE VGEYQGAYKI SQGLLERFGP QRVVDTPISE 

        70         80         90        100        110        120 
HGFTGLAVGA AFCGLKPIVE FMSFNFSMQA MDQIVNSAAK TNYMSGGQLG CPIVFRGPNG 

       130        140        150        160        170        180 
AAAGVAAQHS QCFASWYSHV PGIKVVAPYF AADCKGLLKS AIRDPNPVIF LENEIAYGHS 

       190        200        210        220        230        240 
HEVTEEQLSK DSLVELGKAA IVREGKDVTI ITFSLQLKYA LEAAEILLKD NISAEVIDLR 

       250        260        270        280        290        300 
TLRPLDTEAI LKSVKKTNRV VTVEEGWPFC GVGAEITALI DECAFDDLDA PVTRVTAKDV 

       310        320        330 
PLPYAANLES LALPGVEDIV SAVHKVCNYS IA 

References and documentation are available.

Number of amino acids: 332

Molecular weight: 36018.30

Theoretical pI: 4.80

Amino acid composition: 
Ala (A)  37	 11.1%
Arg (R)  12	  3.6%
Asn (N)  10	  3.0%
Asp (D)  16	  4.8%
Cys (C)   7	  2.1%
Gln (Q)  11	  3.3%
Glu (E)  29	  8.7%
Gly (G)  24	  7.2%
His (H)   6	  1.8%
Ile (I)  21	  6.3%
Leu (L)  29	  8.7%
Lys (K)  16	  4.8%
Met (M)   8	  2.4%
Phe (F)  14	  4.2%
Pro (P)  15	  4.5%
Ser (S)  20	  6.0%
Thr (T)  14	  4.2%
Trp (W)   2	  0.6%
Tyr (Y)   9	  2.7%
Val (V)  32	  9.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1608
Hydrogen    H	      2543
Nitrogen    N	       419
Oxygen      O	       487
Sulfur      S	        15

Formula: C1608H2543N419O487S15
Total number of atoms: 5072

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24785
Abs 0.1% (=1 g/l)   0.688, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.678, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.54
This classifies the protein as stable.



Aliphatic index: 97.83

Grand average of hydropathicity (GRAVY): 0.115