ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEYRYAYPNP DMRSESQTED NLQVPSLAFR VAEASPFAVQ PVSGTAFLKL DAALERRTQR 

        70         80         90        100        110        120 
GAGQISSISS PRPRPRPFPP EPFCLGGGGS SCKSSALIQV TDCRIPNTRS TLEVRAGQAE 

       130        140        150        160        170        180 
ARKVATMTSA VLVDIQDEVT CPICLELLTE PLSIDCGHSF CQACITADNK ESMPGQEGQS 

       190        200        210        220        230        240 
RCPVCQTSYW LGNLRPNRHL ANIAERLREV VVGSGKQLKV ILCAHHGEKL QLFCEEDGKL 

       250        260        270        280        290        300 
ICWLCERSQE HRGHHTFLME EIAQEYQEKF QESLKKLRQE QQEAEKLAAV IREKRISWKN 

       310        320        330        340        350        360 
QMEPERHRIQ KQFDHLRSIL DKEEQRQLKK LEEEERRGLN IIAEAEVELV QQSQSLRELI 

       370        380        390        400        410        420 
SDLEHRCQGS ATELLQDVSD VAKRSEFWTL KKPEALPTKL KSMFRAPDLK KMLRVFRELT 

       430        440        450        460        470        480 
DVQSYWVDVT LNPHTANLNL VLSKNRRQVR FVGAQLSGSH LEEHYGCGVL GSQHFSSGKH 

       490        500        510        520        530        540 
YWEVDVAKKT DWILGVCSDS MGPAFSFNQF ANNRNVYSRY QPQSGYWVIG LHHKHEYRAY 

       550        560        570        580        590        600 
EESSTSLLLS MTVPPRRVGV FLDYDAGTVS FYNVTNHGFP IYTFSKYYFP TTLCPYFNPC 

       610 
NCVFPMTLRR PSS 

References and documentation are available.

Number of amino acids: 613

Molecular weight: 69963.51

Theoretical pI: 7.79

Amino acid composition: 
Ala (A)  37	  6.0%
Arg (R)  45	  7.3%
Asn (N)  21	  3.4%
Asp (D)  22	  3.6%
Cys (C)  20	  3.3%
Gln (Q)  38	  6.2%
Glu (E)  52	  8.5%
Gly (G)  32	  5.2%
His (H)  19	  3.1%
Ile (I)  22	  3.6%
Leu (L)  59	  9.6%
Lys (K)  31	  5.1%
Met (M)  11	  1.8%
Phe (F)  25	  4.1%
Pro (P)  33	  5.4%
Ser (S)  52	  8.5%
Thr (T)  28	  4.6%
Trp (W)   8	  1.3%
Tyr (Y)  19	  3.1%
Val (V)  39	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 74
Total number of positively charged residues (Arg + Lys): 76

Atomic composition:

Carbon      C	      3080
Hydrogen    H	      4836
Nitrogen    N	       884
Oxygen      O	       920
Sulfur      S	        31

Formula: C3080H4836N884O920S31
Total number of atoms: 9751

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73560
Abs 0.1% (=1 g/l)   1.051, assuming all pairs of Cys residues form cystines


Ext. coefficient    72310
Abs 0.1% (=1 g/l)   1.034, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.26
This classifies the protein as unstable.



Aliphatic index: 76.02

Grand average of hydropathicity (GRAVY): -0.512