ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEYWHYVETT SSGQPLLREG EKDIFIDQSV GLYHGKSKIL QRQRGRIFLT SQRIIYIDDA 

        70         80         90        100        110        120 
KPTQNSLGLE LDDLAYVNYS SGFLTRSPRL ILFFKDPSSK DELGKSAETA SADVVSTWVC 

       130        140        150        160        170        180 
PICMVSNETQ GEFTKDTLPT PICINCGVPA DYELTKSSIN CSNAIDPNAN PQNQFGVNSE 

       190        200        210        220        230        240 
NICPACTFAN HPQIGNCEIC GHRLPNASKV RSKLNRLNFH DSRVHIELEK NSLARNKSSH 

       250        260        270        280        290        300 
SALSSSSSTG SSTEFVQLSF RKSDGVLFSQ ATERALENIL TEKNKHIFNQ NVVSVNGVDM 

       310        320        330        340        350        360 
RKGASSHEYN NEVPFIETKL SRIGISSLEK SRENQLLNND ILFNNALTDL NKLMSLATSI 

       370        380        390        400        410        420 
ERLYKNSNIT MKTKTLNLQD ESTVNEPKTR RPLLILDREK FLNKELFLDE IAREIYEFTL 

       430        440        450        460        470        480 
SEFKDLNSDT NYMIITLVDL YAMYNKSMRI GTGLISPMEM REACERFEHL GLNELKLVKV 

       490        500        510        520        530        540 
NKRILCVTSE KFDVVKEKLV DLIGDNPGSD LLRLTQILSS NNSKSNWTLG ILMEVLQNCV 

       550        560 
DEGDLLIDKQ LSGIYYYKNS YWPSHI 

References and documentation are available.

Number of amino acids: 566

Molecular weight: 64017.60

Theoretical pI: 5.97

Amino acid composition: 
Ala (A)  21	  3.7%
Arg (R)  27	  4.8%
Asn (N)  47	  8.3%
Asp (D)  30	  5.3%
Cys (C)  12	  2.1%
Gln (Q)  18	  3.2%
Glu (E)  41	  7.2%
Gly (G)  26	  4.6%
His (H)  11	  1.9%
Ile (I)  39	  6.9%
Leu (L)  65	 11.5%
Lys (K)  37	  6.5%
Met (M)  11	  1.9%
Phe (F)  21	  3.7%
Pro (P)  19	  3.4%
Ser (S)  60	 10.6%
Thr (T)  32	  5.7%
Trp (W)   4	  0.7%
Tyr (Y)  17	  3.0%
Val (V)  28	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      2812
Hydrogen    H	      4487
Nitrogen    N	       775
Oxygen      O	       883
Sulfur      S	        23

Formula: C2812H4487N775O883S23
Total number of atoms: 8980

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48080
Abs 0.1% (=1 g/l)   0.751, assuming all pairs of Cys residues form cystines


Ext. coefficient    47330
Abs 0.1% (=1 g/l)   0.739, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.60
This classifies the protein as unstable.



Aliphatic index: 89.72

Grand average of hydropathicity (GRAVY): -0.400