ProtParam
User-provided sequence:
10 20 30 40 50 60
MFAGRNNDNG FWPCTGLQSK PLVIPELKAH NQFAVDSLQG SVPSSPSDET GDYPPTALDM
70 80 90 100 110 120
DTREIIDTFL KIFTGLPHSK SGRKQVLSTM RRVVDNLAVK HELAYKGMIA RLNLEQKGED
130 140 150 160 170 180
VSFIKQVATE LFSDGTTNWG RIASLLTFGA MLCKYQNDRG HSKYVRLVGE EISSYLLTEQ
190 200 210 220
RDWILRNKAW DGFVEFFHVP DTEAAVRNTL LTIGSVATLS AALAYWIR
References and
documentation are available.
Number of amino acids: 228
Molecular weight: 25531.06
Theoretical pI: 6.52
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 1143
Hydrogen H 1786
Nitrogen N 312
Oxygen O 338
Sulfur S 7
Formula: C
1143H
1786N
312O
338S
7
Total number of atoms: 3586
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36565
Abs 0.1% (=1 g/l) 1.432, assuming all pairs of Cys residues form cystines
Ext. coefficient 36440
Abs 0.1% (=1 g/l) 1.427, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.80
This classifies the protein as stable.
Aliphatic index: 86.40
Grand average of hydropathicity (GRAVY): -0.235