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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFAGRNNDNG FWPCTGLQSK PLVIPELKAH NQFAVDSLQG SVPSSPSDET GDYPPTALDM

70 80 90 100 110 120
DTREIIDTFL KIFTGLPHSK SGRKQVLSTM RRVVDNLAVK HELAYKGMIA RLNLEQKGED

130 140 150 160 170 180
VSFIKQVATE LFSDGTTNWG RIASLLTFGA MLCKYQNDRG HSKYVRLVGE EISSYLLTEQ

190 200 210 220
RDWILRNKAW DGFVEFFHVP DTEAAVRNTL LTIGSVATLS AALAYWIR


References and documentation are available.

Number of amino acids: 228 Molecular weight: 25531.06 Theoretical pI: 6.52
Amino acid composition: 
Ala (A) 17 7.5% Arg (R) 13 5.7% Asn (N) 10 4.4% Asp (D) 14 6.1% Cys (C) 2 0.9% Gln (Q) 8 3.5% Glu (E) 12 5.3% Gly (G) 16 7.0% His (H) 5 2.2% Ile (I) 11 4.8% Leu (L) 24 10.5% Lys (K) 12 5.3% Met (M) 5 2.2% Phe (F) 11 4.8% Pro (P) 9 3.9% Ser (S) 17 7.5% Thr (T) 16 7.0% Trp (W) 5 2.2% Tyr (Y) 6 2.6% Val (V) 15 6.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 26 Total number of positively charged residues (Arg + Lys): 25 Atomic composition: Carbon C 1143 Hydrogen H 1786 Nitrogen N 312 Oxygen O 338 Sulfur S 7 Formula: C1143H1786N312O338S7 Total number of atoms: 3586 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 36565 Abs 0.1% (=1 g/l) 1.432, assuming all pairs of Cys residues form cystines Ext. coefficient 36440 Abs 0.1% (=1 g/l) 1.427, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 30.80 This classifies the protein as stable. Aliphatic index: 86.40 Grand average of hydropathicity (GRAVY): -0.235