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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFAKTAAANL TKKGGLSLLS TTARRTKVTL PDLKWDFGAL EPYISGQINE LHYTKHHQTY

70 80 90 100 110 120
VNGFNTAVDQ FQELSDLLAK EPSPANARKM IAIQQNIKFH GGGFTNHCLF WENLAPESQG

130 140 150 160 170 180
GGEPPTGALA KAIDEQFGSL DELIKLTNTK LAGVQGSGWA FIVKNLSNGG KLDVVQTYNQ

190 200 210 220 230
DTVTGPLVPL VAIDAWEHAY YLQYQNKKAD YFKAIWNVVN WKEASRRFDA GKI


References and documentation are available.

Number of amino acids: 233 Molecular weight: 25774.22 Theoretical pI: 8.49
Amino acid composition: 
Ala (A) 24 10.3% Arg (R) 5 2.1% Asn (N) 15 6.4% Asp (D) 11 4.7% Cys (C) 1 0.4% Gln (Q) 13 5.6% Glu (E) 11 4.7% Gly (G) 20 8.6% His (H) 6 2.6% Ile (I) 11 4.7% Leu (L) 23 9.9% Lys (K) 19 8.2% Met (M) 2 0.9% Phe (F) 11 4.7% Pro (P) 9 3.9% Ser (S) 10 4.3% Thr (T) 16 6.9% Trp (W) 6 2.6% Tyr (Y) 8 3.4% Val (V) 12 5.2% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 22 Total number of positively charged residues (Arg + Lys): 24 Atomic composition: Carbon C 1169 Hydrogen H 1799 Nitrogen N 313 Oxygen O 340 Sulfur S 3 Formula: C1169H1799N313O340S3 Total number of atoms: 3624 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 44920 Abs 0.1% (=1 g/l) 1.743, assuming all pairs of Cys residues form cystines Ext. coefficient 44920 Abs 0.1% (=1 g/l) 1.743, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 27.66 This classifies the protein as stable. Aliphatic index: 82.15 Grand average of hydropathicity (GRAVY): -0.346