ProtParam
User-provided sequence:
10 20 30 40 50 60
MFAKTAAANL TKKGGLSLLS TTARRTKVTL PDLKWDFGAL EPYISGQINE LHYTKHHQTY
70 80 90 100 110 120
VNGFNTAVDQ FQELSDLLAK EPSPANARKM IAIQQNIKFH GGGFTNHCLF WENLAPESQG
130 140 150 160 170 180
GGEPPTGALA KAIDEQFGSL DELIKLTNTK LAGVQGSGWA FIVKNLSNGG KLDVVQTYNQ
190 200 210 220 230
DTVTGPLVPL VAIDAWEHAY YLQYQNKKAD YFKAIWNVVN WKEASRRFDA GKI
References and
documentation are available.
Number of amino acids: 233
Molecular weight: 25774.22
Theoretical pI: 8.49
Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 1169
Hydrogen H 1799
Nitrogen N 313
Oxygen O 340
Sulfur S 3
Formula: C
1169H
1799N
313O
340S
3
Total number of atoms: 3624
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44920
Abs 0.1% (=1 g/l) 1.743, assuming all pairs of Cys residues form cystines
Ext. coefficient 44920
Abs 0.1% (=1 g/l) 1.743, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.66
This classifies the protein as stable.
Aliphatic index: 82.15
Grand average of hydropathicity (GRAVY): -0.346