ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFASQSKACD SPSETVHLQS LPNHIEDLKF RNSDVETDDD LHFPGIAAKN KILKLSWWHS 

        70         80         90        100        110        120 
PVDMHYVATE SVQPKKSDKV PSNKIKKVEN NNTLVNGCSK TSARSVPLLK YNRPDQDGDS 

       130        140        150        160        170        180 
EENITSVLEP NVDEIYSKDS ERLVDDSKPC IGLLNPDQED QMKVCGYRRS LMRTGFCWAC 

       190        200        210        220        230        240 
IFLTGGLLRL VLHWWRHLYL YATCSQCSLE EAEQVLVTED YQGKHKMYHV KQIQVLTSSN 

       250        260        270        280        290        300 
LKTLLEKEQQ SIERTHIECD HVENVLQLSV HFTSAQFKKC SSIRIFRCKQ LVYAWNNNTN 

       310        320        330        340        350        360 
RFQRINGLDL NIPCSYYHQQ RGLPVHEQIS RRIVFGDNEI TVPLRDFKTL LFLEVLNPFY 

       370        380        390        400        410        420 
VFQLFSVILW FTYDYYYYAC VILLMSVFGI TVSVLQTKKN QDVLQKTVYN TGNAWVVDHK 

       430        440        450        460        470        480 
GLSKELPTRA IVPGDIIEIP SSGCTLHCDA ILISGNCILD ESMLTGESVP VTKTPLPSKR 

       490        500        510        520        530        540 
DMIFDKTEHA RHTLFCGTKV IQTRYIGSKK VLAFVINTGN ITAKGELIRS ILYPPPVDYK 

       550        560        570        580        590        600 
FEQDSYKFIQ FLAIIACVGF IYTLVTKILR GTDPVKIAVE SLDLITIVVP PALPAAMTVG 

       610        620        630        640        650 
RFYAQKRLKT SEIFCISPRS INVAGSINCC CFDKILKYSI LRERRERAIL AVTKKVSA 

References and documentation are available.

Number of amino acids: 658

Molecular weight: 74944.63

Theoretical pI: 8.54

Amino acid composition: 
Ala (A)  29	  4.4%
Arg (R)  31	  4.7%
Asn (N)  30	  4.6%
Asp (D)  35	  5.3%
Cys (C)  22	  3.3%
Gln (Q)  28	  4.3%
Glu (E)  34	  5.2%
Gly (G)  27	  4.1%
His (H)  18	  2.7%
Ile (I)  50	  7.6%
Leu (L)  65	  9.9%
Lys (K)  47	  7.1%
Met (M)   9	  1.4%
Phe (F)  27	  4.1%
Pro (P)  29	  4.4%
Ser (S)  51	  7.8%
Thr (T)  40	  6.1%
Trp (W)   8	  1.2%
Tyr (Y)  25	  3.8%
Val (V)  53	  8.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 78

Atomic composition:

Carbon      C	      3367
Hydrogen    H	      5326
Nitrogen    N	       900
Oxygen      O	       971
Sulfur      S	        31

Formula: C3367H5326N900O971S31
Total number of atoms: 10595

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    82625
Abs 0.1% (=1 g/l)   1.102, assuming all pairs of Cys residues form cystines


Ext. coefficient    81250
Abs 0.1% (=1 g/l)   1.084, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.93
This classifies the protein as unstable.



Aliphatic index: 95.93

Grand average of hydropathicity (GRAVY): -0.146