ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFASRFDPSQ LTAPAASAPE GIVGTTPPAI VPLKRQATES DNEEYGSHQD SDESSNSSSE 

        70         80         90        100        110        120 
EDEDRMQVDY GASEEDSSEV EEEESKPSTH STVLSRFKQT VSLQERLGAS DIAESKEDEG 

       130        140        150        160        170        180 
IEDEAASTHQ LKQIPQPEFV KNPMNLNTNS LQFKSTGWLN TEKIYYDNSL IKPFSDYANE 

       190        200        210        220        230        240 
LEAKLLQNIC KNFSTNTFPI QSIILDSILP VLNFTLNVSK RNFTRRIGDI LVNAATGSGK 

       250        260        270        280        290        300 
TLAYSIPIVQ TLFKRQINRL RCIIIVPTKL LINQVYTTLT KLTQGTSLIV SIAKLENSLK 

       310        320        330        340        350        360 
DEHKKLSNLE PDILITTPGR LVDHLNMKSI NLKNLKFLII DEADRLLNQS FQGWCPKLMS 

       370        380        390        400        410        420 
HLKTDKLDTL PGNVIKMIFS ATLTTNTEKL NGLNLYKPKL FLKQTDKLYQ LPNKLNEFNI 

       430        440        450        460        470        480 
NIPTAKSVYK PLILLYSICQ FMAHSPIAAK ILIFVKSNES SIRLSKLLQL ICESRSQSSV 

       490        500        510        520        530        540 
LKNLQNLAVS INSVNSNNSK AENKKIVANF SHHSESAGIT ILITTDIMSR GIDINDITQV 

       550        560        570        580        590        600 
INYDPPMSSQ QYVHRVGRTA RANELGSAYN LLVGRGERTF FDDLNKDLDR DGKSVQPLEL 

       610        620 
DFTLLESDSE LYTSSLESLK NYHNNTAQA 

References and documentation are available.

Number of amino acids: 629

Molecular weight: 70361.78

Theoretical pI: 5.88

Amino acid composition: 
Ala (A)  33	  5.2%
Arg (R)  22	  3.5%
Asn (N)  49	  7.8%
Asp (D)  34	  5.4%
Cys (C)   5	  0.8%
Gln (Q)  29	  4.6%
Glu (E)  41	  6.5%
Gly (G)  22	  3.5%
His (H)  11	  1.7%
Ile (I)  49	  7.8%
Leu (L)  75	 11.9%
Lys (K)  45	  7.2%
Met (M)   9	  1.4%
Phe (F)  22	  3.5%
Pro (P)  27	  4.3%
Ser (S)  68	 10.8%
Thr (T)  43	  6.8%
Trp (W)   2	  0.3%
Tyr (Y)  16	  2.5%
Val (V)  27	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 67

Atomic composition:

Carbon      C	      3107
Hydrogen    H	      5002
Nitrogen    N	       842
Oxygen      O	       985
Sulfur      S	        14

Formula: C3107H5002N842O985S14
Total number of atoms: 9950

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35090
Abs 0.1% (=1 g/l)   0.499, assuming all pairs of Cys residues form cystines


Ext. coefficient    34840
Abs 0.1% (=1 g/l)   0.495, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.51
This classifies the protein as unstable.



Aliphatic index: 94.58

Grand average of hydropathicity (GRAVY): -0.373