ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFCRKFKDLK ITGECPFSLL APGQVPTEPI EEVAGVSESC QATLPTCQEF AENAEGSHPQ 

        70         80         90        100        110        120 
RKTSRNRVYL HTLAESIGKL IFPEFERLNL ALQRTLAKHK IKENRNSSEK EDLERIIAEE 

       130        140        150        160        170        180 
AIAAGVPVEA LKDSLGEELF KICYEEDEHI LGVVGGTLKD FLNSFSTLLK QSSHCQEAER 

       190        200        210        220        230        240 
RGRLEDASIL CLDKDQDFLN VYYFFPKRTT ALLLPGIIKA AARILYESHV EVSLMPPCFR 

       250        260        270        280        290        300 
SECTEFVNQP YLLYSVHVKS TKPSLSPGKP QSSLVIPTSL FCKTFPFHFM LDRDLAILQL 

       310        320        330        340        350        360 
GNGIRRLVNK RDFQGKPNFE EFFEILTPKI NQTFSGIMTM LNMQFVIRVR RWDNLVKKSS 

       370        380        390        400        410        420 
RVMDLKGQMI YIVESSAILF LGSPCVDRLE DFTGRGLYLS DIPIHNALRD VVLIGEQARA 

       430        440        450        460        470        480 
QDGLKKRLGK LKATLEHAHQ ALEEEKKKTV DLLCSIFPSE VAQQLWQGQI VQAKKFNEVT 

       490        500        510        520        530        540 
MLFSDIVGFT AICSQCSPLQ VITMLNALYT RFDQQCGELD VYKVETIGDA YCVAGGLHRE 

       550        560        570        580        590        600 
SDTHAVQIAL MALKMMELSN EVMSPHGEPI KMRIGLHSGS VFAGVVGVKM PRYCLFGNNV 

       610        620        630        640        650        660 
TLANKFESCS VPRKINVSPT TYRLLKDCPG FVFTPRSREE LPPNFPSDIP GICHFLDAYQ 

       670        680        690 
HQGPNSKPWF QQKDAEDGNA NFLGKASGVD 

References and documentation are available.

Number of amino acids: 690

Molecular weight: 77538.47

Theoretical pI: 6.65

Amino acid composition: 
Ala (A)  42	  6.1%
Arg (R)  35	  5.1%
Asn (N)  26	  3.8%
Asp (D)  31	  4.5%
Cys (C)  20	  2.9%
Gln (Q)  32	  4.6%
Glu (E)  52	  7.5%
Gly (G)  42	  6.1%
His (H)  17	  2.5%
Ile (I)  39	  5.7%
Leu (L)  75	 10.9%
Lys (K)  45	  6.5%
Met (M)  16	  2.3%
Phe (F)  40	  5.8%
Pro (P)  37	  5.4%
Ser (S)  48	  7.0%
Thr (T)  31	  4.5%
Trp (W)   3	  0.4%
Tyr (Y)  15	  2.2%
Val (V)  44	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 80

Atomic composition:

Carbon      C	      3465
Hydrogen    H	      5483
Nitrogen    N	       935
Oxygen      O	      1009
Sulfur      S	        36

Formula: C3465H5483N935O1009S36
Total number of atoms: 10928

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40100
Abs 0.1% (=1 g/l)   0.517, assuming all pairs of Cys residues form cystines


Ext. coefficient    38850
Abs 0.1% (=1 g/l)   0.501, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.59
This classifies the protein as unstable.



Aliphatic index: 89.01

Grand average of hydropathicity (GRAVY): -0.182