ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFESVNLDEN SPEDRELAKV LSPPGSYLSP ASLDSGSSFT NSGTSTSCFE PKNNLPSLSF 

        70         80         90        100        110        120 
LNARAGSLGG IFNHKQMTSP SNSNIGGENV ESTTSSNDGS NENAGHPTTS EQDQNADHPT 

       130        140        150        160        170        180 
ISQADDNGHS SLTPNPAVTS TVTDKKGNTV KRKYSRNGCS ECKRRRMKCD ETKPTCWQCA 

       190        200        210        220        230        240 
RLNRQCVYVL NPKNKKRRTS NAQRVKEFRK HSTSLDNDHN NARKRQHSSC KAEKKKKVRQ 

       250        260        270        280        290        300 
NLSEDTTDPK PITDNGKNVP LDEIESLEIP NLDLTTTMNG YDVNLLMQNL NDMVNMKLHD 

       310        320        330        340        350        360 
SYLLNEELKG LDLPDLDIPE LLPASNVNSS VPISFLVNNV ITFNTKLSSF KLGGIHDKYL 

       370        380        390        400        410        420 
KIFYYDCLDS IAPFFQNQGN PLRDILLSFA KNEAYLLSSI LATGASIAYR KSNNLEDERN 

       430        440        450        460        470        480 
YCAYLSHCLS LLGEQFKNES NVLNRIEPII LTVIMLAWDC IYSMNSQWRS HLKGVTDLFK 

       490        500        510        520        530        540 
KINAGNSSKV LNVAKCWFKV METFASISTV FGGSLIDNND LDAIFDPYDY QYVDSLKFLN 

       550        560        570        580        590        600 
IMTPLNEFNL LRGHKEDFDL VIKEVFKSLN TIRSTEKNYF SKEEGLFTKK LDYLLLSSQT 

       610        620        630        640        650        660 
SSEKSKDQIS YFNTQKILVE IDKQLDYEFI DKSGIIPSDN QSHPRISNIH DNAIDMVTLK 

       670        680        690        700        710        720 
NGEEVAISWY DISHQTQVLS FLLIVLLKLL GMPKESSTIQ QVVKKIMSFF KFLDSDSPPQ 

       730        740        750        760        770        780 
NSRTCYSNFA VLIAGLNAMD EETRAIVKRY YKINGGKFQK LTEHNLNRLE KVWYGKNQNY 

       790 
RLEEQDVLTW 

References and documentation are available.

Number of amino acids: 790

Molecular weight: 89396.82

Theoretical pI: 6.50

Amino acid composition: 
Ala (A)  31	  3.9%
Arg (R)  29	  3.7%
Asn (N)  73	  9.2%
Asp (D)  51	  6.5%
Cys (C)  14	  1.8%
Gln (Q)  28	  3.5%
Glu (E)  45	  5.7%
Gly (G)  34	  4.3%
His (H)  16	  2.0%
Ile (I)  44	  5.6%
Leu (L)  84	 10.6%
Lys (K)  63	  8.0%
Met (M)  15	  1.9%
Phe (F)  33	  4.2%
Pro (P)  31	  3.9%
Ser (S)  84	 10.6%
Thr (T)  44	  5.6%
Trp (W)   7	  0.9%
Tyr (Y)  26	  3.3%
Val (V)  38	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 96
Total number of positively charged residues (Arg + Lys): 92

Atomic composition:

Carbon      C	      3936
Hydrogen    H	      6208
Nitrogen    N	      1080
Oxygen      O	      1238
Sulfur      S	        29

Formula: C3936H6208N1080O1238S29
Total number of atoms: 12491

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    78115
Abs 0.1% (=1 g/l)   0.874, assuming all pairs of Cys residues form cystines


Ext. coefficient    77240
Abs 0.1% (=1 g/l)   0.864, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.82
This classifies the protein as stable.



Aliphatic index: 81.06

Grand average of hydropathicity (GRAVY): -0.544