ProtParam
User-provided sequence:
10 20 30 40 50 60
MFFESRPIVG VGGGGGACGG LLTCVGRDAE GKGGADTLGR EILELFLWWL LCAGLTEGIC
70 80 90 100 110 120
GLFCGTFGAE APDNGSGGAG DDGTMGIGGA AEDGISGIGG AEDEGILGTA GAGDNGALGM
130 140 150 160 170 180
GGAATDGTAP GIGGALADGE GLVLALLLIC FNFGIPPAKI SPNCGAPIPG IGGADIEGPL
190 200 210 220 230 240
LLTVPELPEA DETTPPPTIG ALLSLVSAFF SFIPFLMSPN RASRPCITDF AGLGALPPNA
250 260 270 280
GGGGGGGGAG APAISTIRYI YGRLLQQTVV RFLVVCFVSY QKLSS
References and
documentation are available.
Number of amino acids: 285
Molecular weight: 27834.73
Theoretical pI: 4.10
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 11
Atomic composition:
Carbon C 1237
Hydrogen H 1941
Nitrogen N 323
Oxygen O 380
Sulfur S 13
Formula: C
1237H
1941N
323O
380S
13
Total number of atoms: 3894
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15970
Abs 0.1% (=1 g/l) 0.574, assuming all pairs of Cys residues form cystines
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 0.556, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.89
This classifies the protein as stable.
Aliphatic index: 93.58
Grand average of hydropathicity (GRAVY): 0.446