ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFFFPKLRKL IGSTVIDHDT KNSSGKEEIM SNSRLALVII NHAFDKVLSL TWHCGILSEI 

        70         80         90        100        110        120 
RSGLMLMFGI FQLMCSLGVI VLLLPIIILD AIDLFLYMCR LLDYGCKLFH YNRSSLPVAD 

       130        140        150        160        170        180 
GKEKTSGPIS GKEEIVIDEE IINMLNESSE SLINHTTAGL EYDISSGSVN KSRRLNSTST 

       190        200        210        220        230 
VTFVKQNKLV NERREDAYYE EEDDDFLSNP NYDKISLIEK SFTSRFEVAC EQKAA 

References and documentation are available.

Number of amino acids: 235

Molecular weight: 26581.55

Theoretical pI: 5.21

Amino acid composition: 
Ala (A)   9	  3.8%
Arg (R)  10	  4.3%
Asn (N)  13	  5.5%
Asp (D)  14	  6.0%
Cys (C)   5	  2.1%
Gln (Q)   3	  1.3%
Glu (E)  19	  8.1%
Gly (G)  12	  5.1%
His (H)   5	  2.1%
Ile (I)  22	  9.4%
Leu (L)  28	 11.9%
Lys (K)  15	  6.4%
Met (M)   7	  3.0%
Phe (F)  12	  5.1%
Pro (P)   5	  2.1%
Ser (S)  26	 11.1%
Thr (T)  10	  4.3%
Trp (W)   1	  0.4%
Tyr (Y)   7	  3.0%
Val (V)  12	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	      1184
Hydrogen    H	      1885
Nitrogen    N	       307
Oxygen      O	       361
Sulfur      S	        12

Formula: C1184H1885N307O361S12
Total number of atoms: 3749

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16180
Abs 0.1% (=1 g/l)   0.609, assuming all pairs of Cys residues form cystines


Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.599, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.98
This classifies the protein as unstable.



Aliphatic index: 101.62

Grand average of hydropathicity (GRAVY): -0.043