ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFFNRLSAGK LLVPLSVVLY ALFVVILPLQ NSFHSSNVLV RGADDVENYG TVIGIDLGTT 

        70         80         90        100        110        120 
YSCVAVMKNG KTEILANEQG NRITPSYVAF TDDERLIGDA AKNQVAANPQ NTIFDIKRLI 

       130        140        150        160        170        180 
GLKYNDRSVQ KDIKHLPFNV VNKDGKPAVE VSVKGEKKVF TPEEISGMIL GKMKQIAEDY 

       190        200        210        220        230        240 
LGTKVTHAVV TVPAYFNDAQ RQATKDAGTI AGLNVLRIVN EPTAAAIAYG LDKSDKEHQI 

       250        260        270        280        290        300 
IVYDLGGGTF DVSLLSIENG VFEVQATSGD THLGGEDFDY KIVRQLIKAF KKKHGIDVSD 

       310        320        330        340        350        360 
NNKALAKLKR EAEKAKRALS SQMSTRIEID SFVDGIDLSE TLTRAKFEEL NLDLFKKTLK 

       370        380        390        400        410        420 
PVEKVLQDSG LEKKDVDDIV LVGGSTRIPK VQQLLESYFD GKKASKGINP DEAVAYGAAV 

       430        440        450        460        470        480 
QAGVLSGEEG VEDIVLLDVN ALTLGIETTG GVMTPLIKRN TAIPTKKSQI FSTAVDNQPT 

       490        500        510        520        530        540 
VMIKVYEGER AMSKDNNLLG KFELTGIPPA PRGVPQIEVT FALDANGILK VSATDKGTGK 

       550        560        570        580        590        600 
SESITITNDK GRLTQEEIDR MVEEAEKFAS EDASIKAKVE SRNKLENYAH SLKNQVNGDL 

       610        620        630        640        650        660 
GEKLEEEDKE TLLDAANDVL EWLDDNFETA IAEDFDEKFE SLSKVAYPIT SKLYGGADGS 

       670        680 
GAADYDDEDE DDDGDYFEHD EL 

References and documentation are available.

Number of amino acids: 682

Molecular weight: 74467.99

Theoretical pI: 4.79

Amino acid composition: 
Ala (A)  57	  8.4%
Arg (R)  20	  2.9%
Asn (N)  34	  5.0%
Asp (D)  59	  8.7%
Cys (C)   1	  0.1%
Gln (Q)  21	  3.1%
Glu (E)  55	  8.1%
Gly (G)  53	  7.8%
His (H)   8	  1.2%
Ile (I)  43	  6.3%
Leu (L)  62	  9.1%
Lys (K)  60	  8.8%
Met (M)   9	  1.3%
Phe (F)  25	  3.7%
Pro (P)  20	  2.9%
Ser (S)  39	  5.7%
Thr (T)  40	  5.9%
Trp (W)   1	  0.1%
Tyr (Y)  18	  2.6%
Val (V)  57	  8.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 114
Total number of positively charged residues (Arg + Lys): 80

Atomic composition:

Carbon      C	      3295
Hydrogen    H	      5280
Nitrogen    N	       874
Oxygen      O	      1063
Sulfur      S	        10

Formula: C3295H5280N874O1063S10
Total number of atoms: 10522

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32320
Abs 0.1% (=1 g/l)   0.434, assuming all pairs of Cys residues form cystines


Ext. coefficient    32320
Abs 0.1% (=1 g/l)   0.434, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.90
This classifies the protein as stable.



Aliphatic index: 92.64

Grand average of hydropathicity (GRAVY): -0.318