ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFFSKVMLTR RILVRGLATA KSSAPKLTDV LIVGGGPAGL TLAASIKNSP QLKDLKTTLV 

        70         80         90        100        110        120 
DMVDLKDKLS DFYNSPPDYF TNRIVSVTPR SIHFLENNAG ATLMHDRIQS YDGLYVTDGC 

       130        140        150        160        170        180 
SKATLDLARD SMLCMIEIIN IQASLYNRIS QYDSKKDSID IIDNTKVVNI KHSDPNDPLS 

       190        200        210        220        230        240 
WPLVTLSNGE VYKTRLLVGA DGFNSPTRRF SQIPSRGWMY NAYGVVASMK LEYPPFKLRG 

       250        260        270        280        290        300 
WQRFLPTGPI AHLPMPENNA TLVWSSSERL SRLLLSLPPE SFTALINAAF VLEDADMNYY 

       310        320        330        340        350        360 
YRTLEDGSMD TDKLIEDIKF RTEEIYATLK DESDIDEIYP PRVVSIIDKT RARFPLKLTH 

       370        380        390        400        410        420 
ADRYCTDRVA LVGDAAHTTH PLAGQGLNMG QTDVHGLVYA LEKAMERGLD IGSSLSLEPF 

       430        440        450        460        470 
WAERYPSNNV LLGMADKLFK LYHTNFPPVV ALRTFGLNLT NKIGPVKNMI IDTLGGNEK 

References and documentation are available.

Number of amino acids: 479

Molecular weight: 53527.45

Theoretical pI: 6.52

Amino acid composition: 
Ala (A)  32	  6.7%
Arg (R)  26	  5.4%
Asn (N)  25	  5.2%
Asp (D)  36	  7.5%
Cys (C)   3	  0.6%
Gln (Q)   8	  1.7%
Glu (E)  19	  4.0%
Gly (G)  28	  5.8%
His (H)   9	  1.9%
Ile (I)  29	  6.1%
Leu (L)  57	 11.9%
Lys (K)  27	  5.6%
Met (M)  15	  3.1%
Phe (F)  17	  3.5%
Pro (P)  28	  5.8%
Ser (S)  36	  7.5%
Thr (T)  32	  6.7%
Trp (W)   5	  1.0%
Tyr (Y)  19	  4.0%
Val (V)  28	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2398
Hydrogen    H	      3794
Nitrogen    N	       640
Oxygen      O	       710
Sulfur      S	        18

Formula: C2398H3794N640O710S18
Total number of atoms: 7560

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55935
Abs 0.1% (=1 g/l)   1.045, assuming all pairs of Cys residues form cystines


Ext. coefficient    55810
Abs 0.1% (=1 g/l)   1.043, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.37
This classifies the protein as stable.



Aliphatic index: 93.65

Grand average of hydropathicity (GRAVY): -0.187