ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFGPFKLTSP VAGGLLWKIP WRMSTHQKTR QRERLRNVDQ VIKQLTLGLH VQRCQDKGLT 

        70         80         90        100        110        120 
YQEAMESKKK YKPRSKSLRL LNKPSVFPKE NQMSSKDKYW TFDKKAVGYR KGIHKVPKWT 

       130 
KISIRKAPKF F 

References and documentation are available.

Number of amino acids: 131

Molecular weight: 15520.37

Theoretical pI: 10.69

Amino acid composition: 
Ala (A)   4	  3.1%
Arg (R)  10	  7.6%
Asn (N)   3	  2.3%
Asp (D)   4	  3.1%
Cys (C)   1	  0.8%
Gln (Q)   8	  6.1%
Glu (E)   4	  3.1%
Gly (G)   7	  5.3%
His (H)   3	  2.3%
Ile (I)   5	  3.8%
Leu (L)  11	  8.4%
Lys (K)  22	 16.8%
Met (M)   4	  3.1%
Phe (F)   6	  4.6%
Pro (P)   8	  6.1%
Ser (S)   9	  6.9%
Thr (T)   7	  5.3%
Trp (W)   4	  3.1%
Tyr (Y)   4	  3.1%
Val (V)   7	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 8
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	       707
Hydrogen    H	      1138
Nitrogen    N	       204
Oxygen      O	       179
Sulfur      S	         5

Formula: C707H1138N204O179S5
Total number of atoms: 2233

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27960
Abs 0.1% (=1 g/l)   1.802, assuming all pairs of Cys residues form cystines


Ext. coefficient    27960
Abs 0.1% (=1 g/l)   1.802, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.40
This classifies the protein as stable.



Aliphatic index: 66.18

Grand average of hydropathicity (GRAVY): -0.882