ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFGSKRLKNV KEAFKSLKGQ NSETPIENSK ASFKSKNSKT STISKDAKSS SSLKIPISSN 

        70         80         90        100        110        120 
NKNKIFSLAE TNKYGMSSKP IAAAFDFTQN LLAIATVTGE VHIYGQQQVE VVIKLEDRSA 

       130        140        150        160        170        180 
IKEMRFVKGI YLVVINAKDT VYVLSLYSQK VLTTVFVPGK ITSIDTDASL DWMLIGLQNG 

       190        200        210        220        230        240 
SMIVYDIDRD QLSSFKLDNL QKSSFFPAAR LSPIVSIQWN PRDIGTVLIS YEYVTLTYSL 

       250        260        270        280        290        300 
VENEIKQSFI YELPPFAPGG DFSEKTNEKR TPKVIQSLYH PNSLHIITIH EDNSLVFWDA 

       310        320        330        340        350        360 
NSGHMIMART VFETEINVPQ PDYIRDSSTN AAKISKVYWM CENNPEYTSL LISHKSISRG 

       370        380        390        400        410        420 
DNQSLTMIDL GYTPRYSITS YEGMKNYYAN PKQMKIFPLP TNVPIVNILP IPRQSPYFAG 

       430        440        450        460        470        480 
CHNPGLILLI LGNGEIETML YPSGIFTDKA SLFPQNLSWL RPLATTSMAA SVPNKLWLGA 

       490        500        510        520        530        540 
LSAAQNKDYL LKGGVRTKRQ KLPAEYGTAF ITGHSNGSVR IYDASHGDIQ DNASFEVNLS 

       550        560        570        580        590        600 
RTLNKAKELA VDKISFAAET LELAVSIETG DVVLFKYEVN QFYSVENRPE SGDLEMNFRR 

       610        620        630        640        650        660 
FSLNNTNGVL VDVRDRAPTG VRQGFMPSTA VHANKGKTSA INNSNIGFVG IAYAAGSLML 

       670        680        690        700        710        720 
IDRRGPAIIY MENIREISGA QSACVTCIEF VIMEYGDDGY SSILMVCGTD MGEVITYKIL 

       730        740        750        760        770        780 
PASGGKFDVQ LMDITNVTSK GPIHKIDAFS KETKSSCLAT IPKMQNLSKG LCIPGIVLIT 

       790        800        810        820        830        840 
GFDDIRLITL GKSKSTHKGF KYPLAATGLS YISTVEKNND RKNLTVIITL EINGHLRVFT 

       850        860        870        880        890        900 
IPDFKEQMSE HIPFPIAAKY ITESSVLRNG DIAIRVSEFQ ASLFSTVKEQ DTLAPVSDTL 

       910        920        930        940        950        960 
YINGIRIPYR PQVNSLQWAR GTVYCTPAQL NELLGGVNRP ASKYKESIIA EGSFSERSSD 

       970        980        990       1000       1010       1020 
DNNANHPEHQ YTKPTRKGRN SSYGVLRNVS RAVETRWDAV EDRFNDYATA MGETMNEAVE 

      1030 
QTGKDVMKGA LGF 

References and documentation are available.

Number of amino acids: 1033

Molecular weight: 114512.65

Theoretical pI: 8.57

Amino acid composition: 
Ala (A)  68	  6.6%
Arg (R)  42	  4.1%
Asn (N)  65	  6.3%
Asp (D)  49	  4.7%
Cys (C)   8	  0.8%
Gln (Q)  33	  3.2%
Glu (E)  55	  5.3%
Gly (G)  65	  6.3%
His (H)  16	  1.5%
Ile (I)  85	  8.2%
Leu (L)  81	  7.8%
Lys (K)  69	  6.7%
Met (M)  26	  2.5%
Phe (F)  42	  4.1%
Pro (P)  48	  4.6%
Ser (S) 100	  9.7%
Thr (T)  67	  6.5%
Trp (W)   8	  0.8%
Tyr (Y)  41	  4.0%
Val (V)  65	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 111

Atomic composition:

Carbon      C	      5110
Hydrogen    H	      8082
Nitrogen    N	      1366
Oxygen      O	      1548
Sulfur      S	        34

Formula: C5110H8082N1366O1548S34
Total number of atoms: 16140

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   105590
Abs 0.1% (=1 g/l)   0.922, assuming all pairs of Cys residues form cystines


Ext. coefficient   105090
Abs 0.1% (=1 g/l)   0.918, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.93
This classifies the protein as stable.



Aliphatic index: 87.50

Grand average of hydropathicity (GRAVY): -0.226