ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFGVSRGAFP SATTQPFGST GSTFGGQQQQ QQPVANTSAF GLSQQTNTTQ APAFGNFGNQ 

        70         80         90        100        110        120 
TSNSPFGMSG STTANGTPFG QSQLTNNNAS GSIFGGMGNN TALSAGSASV VPNSTAGTSI 

       130        140        150        160        170        180 
KPFTTFEEKD PTTGVINVFQ SITCMPEYRN FSFEELRFQD YQAGRKFGTS QNGTGTTFNN 

       190        200        210        220        230        240 
PQGTTNTGFG IMGNNNSTTS ATTGGLFGQK PATGMFGTGT GSGGGFGSGA TNSTGLFGSS 

       250        260        270        280        290        300 
TNLSGNSAFG ANKPATSGGL FGNTTNNPTN GTNNTGLFGQ QNSNTNGGLF GQQQNSFGAN 

       310        320        330        340        350        360 
NVSNGGAFGQ VNRGAFPQQQ TQQGSGGIFG QSNANANGGA FGQQQGTGAL FGAKPASGGL 

       370        380        390        400        410        420 
FGQSAGSKAF GMNTNPTGTT GGLFGQTNQQ QSGGGLFGQQ QNSNAGGLFG QNNQSQNQSG 

       430        440        450        460        470        480 
LFGQQNSSNA FGQPQQQGGL FGSKPAGGLF GQQQGASTFA SGNAQNNSIF GQNNQQQQST 

       490        500        510        520        530        540 
GGLFGQQNNQ SQSQPGGLFG QTNQNNNQPF GQNGLQQPQQ NNSLFGAKPT GFGNTSLFSN 

       550        560        570        580        590        600 
STTNQSNGIS GNNLQQQSGG LFQNKQQPAS GGLFGSKPSN TVGGGLFGNN QVANQNNPAS 

       610        620        630        640        650        660 
TSGGLFGSKP ATGSLFGGTN STAPNASSGG IFGSNNASNT AATTNSTGLF GNKPVGAGAS 

       670        680        690        700        710        720 
TSAGGLFGNN NNSSLNNSNG STGLFGSNNT SQSTNAGGLF QNNTSTNTSG GGLFSQPSQS 

       730        740        750        760        770        780 
MAQSQNALQQ QQQQQRLQIQ NNNPYGTNEL FSKATVTNTV SYPIQPSATK IKADERKKAS 

       790        800        810        820        830        840 
LTNAYKMIPK TLFTAKLKTN NSVMDKAQIK VDPKLSISID KKNNQIAISN QQEENLDESI 

       850        860        870        880        890        900 
LKASELLFNP DKRSFKNLIN NRKMLIASEE KNNGSQNNDM NFKSKSEEQE TILGKPKMDE 

       910        920        930        940        950        960 
KETANGGERM VLSSKNDGED SATKHHSRNM DEENKENVAD LQKQEYSEDD KKAVFADVAE 

       970        980        990       1000       1010       1020 
KDASFINENY YISPSLDTLS SYSLLQLRKV PHLVVGHKSY GKIEFLEPVD LAGIPLTSLG 

      1030       1040       1050       1060       1070       1080 
GVIITFEPKT CIIYANLPNR PKRGEGINVR ARITCFNCYP VDKSTRKPIK DPNHQLVKRH 

      1090       1100       1110 
IERLKKNPNS KFESYDADSG TYVFIVNHAA EQT 

References and documentation are available.

Number of amino acids: 1113

Molecular weight: 116234.19

Theoretical pI: 9.36

Amino acid composition: 
Ala (A)  75	  6.7%
Arg (R)  19	  1.7%
Asn (N) 136	 12.2%
Asp (D)  24	  2.2%
Cys (C)   4	  0.4%
Gln (Q) 110	  9.9%
Glu (E)  34	  3.1%
Gly (G) 156	 14.0%
His (H)   7	  0.6%
Ile (I)  35	  3.1%
Leu (L)  64	  5.8%
Lys (K)  56	  5.0%
Met (M)  15	  1.3%
Phe (F)  73	  6.6%
Pro (P)  48	  4.3%
Ser (S) 119	 10.7%
Thr (T)  95	  8.5%
Trp (W)   0	  0.0%
Tyr (Y)  14	  1.3%
Val (V)  29	  2.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 75

Atomic composition:

Carbon      C	      4975
Hydrogen    H	      7732
Nitrogen    N	      1486
Oxygen      O	      1704
Sulfur      S	        19

Formula: C4975H7732N1486O1704S19
Total number of atoms: 15916

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21110
Abs 0.1% (=1 g/l)   0.182, assuming all pairs of Cys residues form cystines


Ext. coefficient    20860
Abs 0.1% (=1 g/l)   0.179, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.18
This classifies the protein as stable.



Aliphatic index: 48.98

Grand average of hydropathicity (GRAVY): -0.727