ProtParam
User-provided sequence:
10 20 30 40 50 60
MFHGAKGPLL IERIGHLLSI NYGEKEERKR WAMQGISYLQ EVQCTSTPYL EILVEESGLR
70 80 90 100 110 120
PVSLLNSQLV GKPHFSLLGG FDENIARDII SHNFQNAIFQ MESEEVPLTK RYQHLEKITQ
130 140 150 160 170 180
ISLLCKNFKG IEEIEYNVKN IIQGRKNFDM LNSMEKDRIS HEVVQDDSFS LLRIQMLLCV
190 200 210 220 230 240
SYFLQERYFD CCTKFFTMMT SEPLTLKVLS EHLDCMNFIS KEEFIMMVNI SVLISIPLDN
250 260 270 280 290 300
YDDFIYLSDL KQFFQMTPLL VNCLELLINT NFNKFFKIWH GEINKICMES LFLEPSWSSS
310 320 330 340 350 360
AAVIMRCKIY FFYLRISKKL QFSYLSSTLG IDLEDIKEEL TKLIISGQLN FEIDGDVIHF
370 380 390 400 410 420
EDSSILQSIV NEISRNGTMI NEVIDKLKNE NTDLKDIIQG NPLMYSGGNN TATIINNESS
430 440
DDMDIDEVND RSDISDSEGG LFEC
References and
documentation are available.
Number of amino acids: 444
Molecular weight: 51287.86
Theoretical pI: 4.75
Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 2293
Hydrogen H 3595
Nitrogen N 581
Oxygen O 695
Sulfur S 27
Formula: C
2293H
3595N
581O
695S
27
Total number of atoms: 7191
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36495
Abs 0.1% (=1 g/l) 0.712, assuming all pairs of Cys residues form cystines
Ext. coefficient 35870
Abs 0.1% (=1 g/l) 0.699, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.33
This classifies the protein as unstable.
Aliphatic index: 99.41
Grand average of hydropathicity (GRAVY): -0.121