ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFIGIAYLGG DRQMDELPDH LVWDILSKLH TTDDRNSLSL SCKRFFSLDN EQRYSLRIGC 

        70         80         90        100        110        120 
GLVPASDALL SLCRRFPNLS KVEIIYSGWM SKLGKQVDDQ GLLVLTTNCH SLTDLTLSFC 

       130        140        150        160        170        180 
TFITDVGIGH LSSCPELSSL KLNFAPRITG CGVLSLAVGC KKLRRLHLIR CLNVASVEWL 

       190        200        210        220        230        240 
EYFGKLETLE ELCIKNCRAI GEGDLIKLRN SWRKLTSLQF EVDANYRYMK VYDQLDVERW 

       250        260        270        280        290        300 
PKQLVPCDSL VELSLGNCII APGRGLACVL RNCKNLEKLH LDMCTGVSDS DIIALVQKAS 

       310        320        330        340        350        360 
HLRSISLRVP SDFTLPLLNN ITLRLTDESL SAIAQHCSKL ESFKISFSDG EFPSLFSFTL 

       370        380        390        400        410        420 
QGIITLIQKC PVRELSLDHV CVFNDMGMEA LCSAQKLEIL ELVHCQEVSD EGLILVSQFP 

       430        440        450        460        470        480 
SLNVLKLSKC LGVTDDGMRP LVGSHKLELL VVEDCPQVSR RGVHGAATSV SFKQDLSWMY 

References and documentation are available.

Number of amino acids: 480

Molecular weight: 53598.38

Theoretical pI: 6.20

Amino acid composition: 
Ala (A)  18	  3.8%
Arg (R)  25	  5.2%
Asn (N)  16	  3.3%
Asp (D)  30	  6.2%
Cys (C)  23	  4.8%
Gln (Q)  16	  3.3%
Glu (E)  25	  5.2%
Gly (G)  29	  6.0%
His (H)  12	  2.5%
Ile (I)  26	  5.4%
Leu (L)  79	 16.5%
Lys (K)  25	  5.2%
Met (M)   9	  1.9%
Phe (F)  18	  3.8%
Pro (P)  15	  3.1%
Ser (S)  48	 10.0%
Thr (T)  19	  4.0%
Trp (W)   6	  1.2%
Tyr (Y)   8	  1.7%
Val (V)  33	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2377
Hydrogen    H	      3832
Nitrogen    N	       642
Oxygen      O	       698
Sulfur      S	        32

Formula: C2377H3832N642O698S32
Total number of atoms: 7581

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    46295
Abs 0.1% (=1 g/l)   0.864, assuming all pairs of Cys residues form cystines


Ext. coefficient    44920
Abs 0.1% (=1 g/l)   0.838, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.65
This classifies the protein as stable.



Aliphatic index: 109.00

Grand average of hydropathicity (GRAVY): 0.119