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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFISRRCRIK GFTLKNLLWF RSSSTRFVST ESPDASAITK PDGIFNYSLL ENRTYIRIRG

70 80 90 100 110 120
PDTVKFLNGL VTSKLLPHFI KKNLTTVEEN EVPTEEGTTK VDPIIPVPEF DARLGNWGLY

130 140 150 160 170 180
NEKGIQGPYI SRFGLYSAFL NGKGKLITDT IIYPTPVTVS EQISNYPEYL LELHGNVVDK

190 200 210 220 230 240
ILHVLQTHKL ANKIKFEKID HSSLKTWDVE VQFPNLPKDI ENPWFDNLLD PMALPKNSID

250 260 270 280 290 300
ANNFAVNVLN SLFNSDPRIL GIYVERRTES MSRHYSTFPQ SFRVVTSEQV DDLSKLFNFN

310 320 330 340 350 360
VFDFPFQVNK KASVQVREIR FQKGLIDSTE DYISETLLPL ELNFDFFPNT ISTNKGCYVG

370 380 390 400 410 420
QELTARTYAT GILRKRLVPV KLDNYQLLDT DPERKYAEFH IDNVVEKSLA ENEPTLNPFT

430 440 450 460 470 480
NKPPERTKRK QRPAGLLISN EGLYGVALLR TEHFSAAFSS DEPVEFYITT TKGENIKITP

490
QKPFWFSDWK NNNGPHK


References and documentation are available.

Number of amino acids: 497 Molecular weight: 57054.92 Theoretical pI: 7.70
Amino acid composition: 
Ala (A) 17 3.4% Arg (R) 25 5.0% Asn (N) 37 7.4% Asp (D) 26 5.2% Cys (C) 2 0.4% Gln (Q) 13 2.6% Glu (E) 34 6.8% Gly (G) 23 4.6% His (H) 9 1.8% Ile (I) 32 6.4% Leu (L) 49 9.9% Lys (K) 36 7.2% Met (M) 3 0.6% Phe (F) 33 6.6% Pro (P) 33 6.6% Ser (S) 34 6.8% Thr (T) 37 7.4% Trp (W) 6 1.2% Tyr (Y) 17 3.4% Val (V) 31 6.2% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 60 Total number of positively charged residues (Arg + Lys): 61 Atomic composition: Carbon C 2597 Hydrogen H 4022 Nitrogen N 682 Oxygen O 756 Sulfur S 5 Formula: C2597H4022N682O756S5 Total number of atoms: 8062 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 58455 Abs 0.1% (=1 g/l) 1.025, assuming all pairs of Cys residues form cystines Ext. coefficient 58330 Abs 0.1% (=1 g/l) 1.022, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 37.18 This classifies the protein as stable. Aliphatic index: 85.07 Grand average of hydropathicity (GRAVY): -0.433