ProtParam
User-provided sequence:
10 20 30 40 50 60
MFISRRCRIK GFTLKNLLWF RSSSTRFVST ESPDASAITK PDGIFNYSLL ENRTYIRIRG
70 80 90 100 110 120
PDTVKFLNGL VTSKLLPHFI KKNLTTVEEN EVPTEEGTTK VDPIIPVPEF DARLGNWGLY
130 140 150 160 170 180
NEKGIQGPYI SRFGLYSAFL NGKGKLITDT IIYPTPVTVS EQISNYPEYL LELHGNVVDK
190 200 210 220 230 240
ILHVLQTHKL ANKIKFEKID HSSLKTWDVE VQFPNLPKDI ENPWFDNLLD PMALPKNSID
250 260 270 280 290 300
ANNFAVNVLN SLFNSDPRIL GIYVERRTES MSRHYSTFPQ SFRVVTSEQV DDLSKLFNFN
310 320 330 340 350 360
VFDFPFQVNK KASVQVREIR FQKGLIDSTE DYISETLLPL ELNFDFFPNT ISTNKGCYVG
370 380 390 400 410 420
QELTARTYAT GILRKRLVPV KLDNYQLLDT DPERKYAEFH IDNVVEKSLA ENEPTLNPFT
430 440 450 460 470 480
NKPPERTKRK QRPAGLLISN EGLYGVALLR TEHFSAAFSS DEPVEFYITT TKGENIKITP
490
QKPFWFSDWK NNNGPHK
References and
documentation are available.
Number of amino acids: 497
Molecular weight: 57054.92
Theoretical pI: 7.70
Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 61
Atomic composition:
Carbon C 2597
Hydrogen H 4022
Nitrogen N 682
Oxygen O 756
Sulfur S 5
Formula: C
2597H
4022N
682O
756S
5
Total number of atoms: 8062
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 58455
Abs 0.1% (=1 g/l) 1.025, assuming all pairs of Cys residues form cystines
Ext. coefficient 58330
Abs 0.1% (=1 g/l) 1.022, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.18
This classifies the protein as stable.
Aliphatic index: 85.07
Grand average of hydropathicity (GRAVY): -0.433