ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFKEEDSLRK GQNVAAWKTL LAGAVSGLLA RSITAPMDTI KIRLQLTPAN GLKPFGSQVM 

        70         80         90        100        110        120 
EVARSMIKNE GIRSFWKGNI PGSLLYVTYG SAQFSSYSLF NRYLTPFGLE ARLHSLVVGA 

       130        140        150        160        170        180 
FAGITSSIVS YPFDVLRTRL VANNQMHSMS ITREVRDIWK LEGLPGFFKG SIASMTTITL 

       190        200        210        220        230        240 
TASIMFGTYE TIRIYCDENE KTTAAHKKWE LATLNHSAGT IGGVIAKIIT FPLETIRRRM 

       250        260        270        280        290        300 
QFMNSKHLEK FSRHSSVYGS YKGYGFARIG LQILKQEGVS SLYRGILVAL SKTIPTTFVS 

       310 
FWGYETAIHY LRMY 

References and documentation are available.

Number of amino acids: 314

Molecular weight: 35136.82

Theoretical pI: 9.94

Amino acid composition: 
Ala (A)  23	  7.3%
Arg (R)  19	  6.1%
Asn (N)  10	  3.2%
Asp (D)   5	  1.6%
Cys (C)   1	  0.3%
Gln (Q)   8	  2.5%
Glu (E)  15	  4.8%
Gly (G)  26	  8.3%
His (H)   7	  2.2%
Ile (I)  25	  8.0%
Leu (L)  30	  9.6%
Lys (K)  18	  5.7%
Met (M)  11	  3.5%
Phe (F)  17	  5.4%
Pro (P)   9	  2.9%
Ser (S)  30	  9.6%
Thr (T)  25	  8.0%
Trp (W)   5	  1.6%
Tyr (Y)  14	  4.5%
Val (V)  16	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1597
Hydrogen    H	      2512
Nitrogen    N	       426
Oxygen      O	       442
Sulfur      S	        12

Formula: C1597H2512N426O442S12
Total number of atoms: 4989

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48360
Abs 0.1% (=1 g/l)   1.376, assuming all pairs of Cys residues form cystines


Ext. coefficient    48360
Abs 0.1% (=1 g/l)   1.376, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.14
This classifies the protein as stable.



Aliphatic index: 90.41

Grand average of hydropathicity (GRAVY): 0.019