ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFKKSRHLKN VSNAIKSARV HDVSNGINSK FFDTKKICTY GINGRITVTT FDYTQSLLAV 

        70         80         90        100        110        120 
ATTAGEIHVY GQKQIEVVFT LKNRPQIKHM RFIKGIYLIA VDEKSNIIVL SVHSKQILTT 

       130        140        150        160        170        180 
VFCPNSITCF ETDPPLDWML IGLESGSILI YDVDRNQMSK LKIENFQKSV FLPKERLSPV 

       190        200        210        220        230        240 
ISIQWNPRDI GTILISYEHI TVIYSFIDYK VKQHFFYQLE PYAPGGDLST NIEKKRTPKV 

       250        260        270        280        290        300 
IQSLYHPNSL HILTVHEDNA LVFWDVNSGK LIHARSIFET HVNFPNPALK DCSFTETPAI 

       310        320        330        340        350        360 
FKVSWLCQRN PEYTSLLIAT KATENPCLPQ EITMIDLGGT PMYSVTSFDA MSKYYAKPVQ 

       370        380        390        400        410        420 
QKLFSLIGKA PLINFLPLPK ASPYFGGCHD TNLILLLLED GELETLIYPA GSFSSKASIF 

       430        440        450        460        470        480 
PRSLAWVRPT VTTCIAQSVQ KNLWLGMMTI AQSESFLKGG IPATRNIRRH ETRSALLTGH 

       490        500        510        520        530        540 
SNGSVRIWDA SHSEVTDNAV FEVNTAKVLN RATNLAIKNI SFASETLELA VSSEVGDVIL 

       550        560        570        580        590        600 
FKFETNKFYG QLPKSDALQL KFSRFSLDDS KTILVDVSDR GPTNVKQGFI PSTVIHAKKG 

       610        620        630        640        650        660 
AVSAIMNSNI GFVAVGFIEG TLIILDRRGP AIIFNENIRV ISKAGSSYVS TVHFCVMEYG 

       670        680        690        700        710        720 
DDGFSSILML CGTDIGELMT FKILPATNGR FEVKFTDATK TNNQGKILGI NSFAKDTGYS 

       730        740        750        760        770        780 
CSATISKMQG LSKGIAIPGF VTISGANDIR LVSPGKSKDT HALFKYPIAT SGLSFIPIID 

       790        800        810        820        830        840 
GKGERKLSTI MIVLLINGDI KVLTVPELKE VKNLRCPVPL SAQYVENSSI LENVDIVIRT 

       850        860        870        880        890        900 
GKFQASLISV LNESATGSNH TADISQHTPI DTLYNPDLKI GYRPQVNSLQ WARGTIYCTP 

       910        920        930        940        950        960 
YQLDELLGGI ERPESKYEES AIARGCISSS SSNAARKLPP GTKDHRYARP VRSSGRSNGY 

       970        980        990       1000       1010 
GVLKSVSRAI ETRLDTVETT INDYATTMGQ TMNDAMEETG RDMMKSAVGF 

References and documentation are available.

Number of amino acids: 1010

Molecular weight: 111678.44

Theoretical pI: 8.93

Amino acid composition: 
Ala (A)  60	  5.9%
Arg (R)  41	  4.1%
Asn (N)  52	  5.1%
Asp (D)  46	  4.6%
Cys (C)  14	  1.4%
Gln (Q)  30	  3.0%
Glu (E)  47	  4.7%
Gly (G)  65	  6.4%
His (H)  22	  2.2%
Ile (I)  92	  9.1%
Leu (L)  86	  8.5%
Lys (K)  67	  6.6%
Met (M)  20	  2.0%
Phe (F)  47	  4.7%
Pro (P)  47	  4.7%
Ser (S)  95	  9.4%
Thr (T)  75	  7.4%
Trp (W)   8	  0.8%
Tyr (Y)  31	  3.1%
Val (V)  65	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 93
Total number of positively charged residues (Arg + Lys): 108

Atomic composition:

Carbon      C	      5012
Hydrogen    H	      7962
Nitrogen    N	      1334
Oxygen      O	      1480
Sulfur      S	        34

Formula: C5012H7962N1334O1480S34
Total number of atoms: 15822

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    91065
Abs 0.1% (=1 g/l)   0.815, assuming all pairs of Cys residues form cystines


Ext. coefficient    90190
Abs 0.1% (=1 g/l)   0.808, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.55
This classifies the protein as stable.



Aliphatic index: 93.34

Grand average of hydropathicity (GRAVY): -0.079