ProtParam
User-provided sequence:
10 20 30 40 50 60
MFKKYKNQDL DMAFWWKKNP KTPSDYARLI IEQLNKFSSP SLTQDNKRKV QEECTKYLIG
70 80 90 100 110 120
TKHFIVGDTD PHPTPEAIDE LYTAMHRADV FYELLLHFVD LEFEARRECM LIFSICLGYS
130 140 150 160 170 180
KDNKFVTVDY LVSQPKTISL MLRTAEVALQ QKGCQDIFLT VGNMIIECIK YEQLCRIILK
190 200 210 220 230 240
DPQLWKFFEF AKLGNFEIST ESLQILSAAF TAHPKLVSKE FFSNEINIIR FIKCINKLMA
250 260 270 280 290 300
HGSYVTKRQS TKLLASLIVI RSNNALMNIY INSPENLKLI MTLMTDKSKN LQLEAFNVFK
310 320 330 340 350 360
VMVANPRKSK PVFDILVKNR DKLLTYFKTF GLDSQDSTFL DEREFIVQEI DSLPRIISST
370 380 390
TEVSNNNASS SNVASITSPS SVMNNQSSIL THSTSPDSR
References and
documentation are available.
Number of amino acids: 399
Molecular weight: 45853.05
Theoretical pI: 8.64
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 49
Atomic composition:
Carbon C 2064
Hydrogen H 3272
Nitrogen N 536
Oxygen O 603
Sulfur S 19
Formula: C
2064H
3272N
536O
603S
19
Total number of atoms: 6494
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 33265
Abs 0.1% (=1 g/l) 0.725, assuming all pairs of Cys residues form cystines
Ext. coefficient 32890
Abs 0.1% (=1 g/l) 0.717, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.67
This classifies the protein as unstable.
Aliphatic index: 92.36
Grand average of hydropathicity (GRAVY): -0.188