ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFKLKNGQEI PKLALGTYEA KGDQLFAAVD EALKVGYRSF DTAKYYENEK DLGLALKTLL 

        70         80         90        100        110        120 
PRHNICSEDI YLTSKVFPYS SKNAAELIRK DVNESLELLD RKYLDLVLVH YPRPLDTEDL 

       130        140        150        160        170        180 
NENNKMYRKD TWIALEKLHA EGKIRSIGVS NYEPHHIEEM RSYITIEPQV NQIEYHPHFQ 

       190        200        210        220        230        240 
RKVLRAYCNK NEILFQAFSP LGRGNKTLLG DSTMERIALC HKTTVANVIL AWIMKGKYGV 

       250        260        270        280 
VAKSVTPSRV AENYTSLSLE LSDDEFEKIN GLNLETPYVE DRGWFVL 

References and documentation are available.

Number of amino acids: 287

Molecular weight: 33040.79

Theoretical pI: 6.27

Amino acid composition: 
Ala (A)  18	  6.3%
Arg (R)  14	  4.9%
Asn (N)  17	  5.9%
Asp (D)  15	  5.2%
Cys (C)   3	  1.0%
Gln (Q)   6	  2.1%
Glu (E)  26	  9.1%
Gly (G)  14	  4.9%
His (H)   8	  2.8%
Ile (I)  16	  5.6%
Leu (L)  35	 12.2%
Lys (K)  24	  8.4%
Met (M)   5	  1.7%
Phe (F)   9	  3.1%
Pro (P)  11	  3.8%
Ser (S)  16	  5.6%
Thr (T)  14	  4.9%
Trp (W)   3	  1.0%
Tyr (Y)  16	  5.6%
Val (V)  17	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1488
Hydrogen    H	      2337
Nitrogen    N	       395
Oxygen      O	       439
Sulfur      S	         8

Formula: C1488H2337N395O439S8
Total number of atoms: 4667

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40465
Abs 0.1% (=1 g/l)   1.225, assuming all pairs of Cys residues form cystines


Ext. coefficient    40340
Abs 0.1% (=1 g/l)   1.221, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.56
This classifies the protein as stable.



Aliphatic index: 92.75

Grand average of hydropathicity (GRAVY): -0.434