ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFKQILGKLP KKTSAKFWDN GESQTLDNNN NQGGGDEVLS QRTSSNGDTS LDCVSSFDVL 

        70         80         90        100        110        120 
PRLRDVSISE KQELFLKKLR LCCLVFDFVA EPQQNFKEKE IKRQTLLEVV DYVISSGNGK 

       130        140        150        160        170        180 
FPESVIQEAT KMISANLFSN PHRQWKNKTP EALDLEEEEG SLNPSWPHLQ IVYEFLLRIV 

       190        200        210        220        230        240 
ASPNTDPKIS KKYIDHTFVL KLLDLFDSED PREREYLKTI LHRIYGRFMV HRPFIRKTMN 

       250        260        270        280        290        300 
NILYDFIFET GKHSGIAEFL EVLGSIINGF ALPLKEEHKL FLTRVLIPLH KLKCLPNYHQ 

       310        320        330        340        350        360 
QLSYCVIQFV EKDCKLADTV IRGMLKYWPV TNSAKEIMFL NELEEILEAT QLTEFERCMV 

       370        380        390        400        410        420 
PLSRQIAQCL SSSHFQVAER ALYLWNNDHV TNLVRQNSRI ILPIVFPALE KNGSSHWNQA 

       430        440        450        460        470 
VKNLTENVLK VLSDTNPDLF EECLHKFQED QQKAEDTKKK NGETWRQLEE IVASMAK 

References and documentation are available.

Number of amino acids: 477

Molecular weight: 55240.41

Theoretical pI: 6.27

Amino acid composition: 
Ala (A)  19	  4.0%
Arg (R)  21	  4.4%
Asn (N)  29	  6.1%
Asp (D)  23	  4.8%
Cys (C)   9	  1.9%
Gln (Q)  24	  5.0%
Glu (E)  42	  8.8%
Gly (G)  17	  3.6%
His (H)  13	  2.7%
Ile (I)  28	  5.9%
Leu (L)  58	 12.2%
Lys (K)  39	  8.2%
Met (M)   8	  1.7%
Phe (F)  26	  5.5%
Pro (P)  20	  4.2%
Ser (S)  33	  6.9%
Thr (T)  22	  4.6%
Trp (W)   7	  1.5%
Tyr (Y)  10	  2.1%
Val (V)  29	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      2483
Hydrogen    H	      3911
Nitrogen    N	       665
Oxygen      O	       726
Sulfur      S	        17

Formula: C2483H3911N665O726S17
Total number of atoms: 7802

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53900
Abs 0.1% (=1 g/l)   0.976, assuming all pairs of Cys residues form cystines


Ext. coefficient    53400
Abs 0.1% (=1 g/l)   0.967, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.41
This classifies the protein as stable.



Aliphatic index: 91.93

Grand average of hydropathicity (GRAVY): -0.395