ProtParam
User-provided sequence:
10 20 30 40 50 60
MFKSLFDAAQ NSTFKSPFTS VNCQSATPTS APTSTAVVTP TLKDVAPRQL TRNHNIAAAA
70 80 90 100 110 120
VAEGDSCEFG SNHYFLLCGL GGIISCGSTH TMVVPLDLVK CRLQVDPAKY KSVFTGFRIS
130 140 150 160 170 180
LAEEGVRGLA KGWAPTFIGY SMQGLCKFGL YEVFKKVYGD AIGEENAFLY RTGLYLAASA
190 200 210 220 230 240
SAEFFADIAL APMEAAKVKI QTTPGFAKTL REALPKMTAQ EGVTAFYKGL VPLWMRQIPY
250 260 270 280 290 300
TMMKFACFER TLELLYKYVV PKPRADCTKG EQLVVTFAAG YIAGVFCAIV SHPADTVVSK
310 320 330 340 350 360
LNQAKGASAL DVAKQLGWSG LWGGLVPRIV MIGTLTAAQW FIYDAVKVFL RMPRPPPPEM
370
PESLKKKLGV TGEQ
References and
documentation are available.
Number of amino acids: 374
Molecular weight: 40553.33
Theoretical pI: 9.14
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1848
Hydrogen H 2890
Nitrogen N 472
Oxygen O 512
Sulfur S 20
Formula: C
1848H
2890N
472O
512S
20
Total number of atoms: 5742
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 47370
Abs 0.1% (=1 g/l) 1.168, assuming all pairs of Cys residues form cystines
Ext. coefficient 46870
Abs 0.1% (=1 g/l) 1.156, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.29
This classifies the protein as stable.
Aliphatic index: 85.08
Grand average of hydropathicity (GRAVY): 0.149