ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFKSTLNSII RRPLKGFQLL RGADSSNTRP QSPRASARDV TEKQILRTPS APTAIPLREI 

        70         80         90        100        110        120 
IYRVPSLFPR PLEDSVKDFR DFIKNEDAFQ TELLKTLPFY PTPSESKTAR LIRTVVDDEG 

       130        140        150        160        170        180 
NYINEFCIRP RKTSVPEADL KHLVFIHGYG AGLGFFIKNF EDIPLLDNEW CIHAIDLPGY 

       190        200        210        220        230        240 
GFSSRPKFPF EYPRDNIHSV QDWFHERIHT WFSKRNLLNR PEKNIVMAHS LGSYLMALYL 

       250        260        270        280        290        300 
QKYKESPSFK KLILCSPAGV SYRDFNNTAS EVEKWKPPPW WYVKLWDRNI SPFTLVRNFR 

       310        320        330        340        350        360 
QLGSKITSGW SYRRFKHILN GDPEQSKRFE ALHRYAYAIF NKRGSGEYLL SFALKCGGEP 

       370        380        390        400        410        420 
RLSLEQQLFD GKKSDILKNS NCDWLWLYGD DDWMDVNGGL RVSRFLKEKL KQKSNVIIVP 

       430        440 
HSGHHLYLDN YKFFNNILTK EMQKI 

References and documentation are available.

Number of amino acids: 445

Molecular weight: 52044.68

Theoretical pI: 9.52

Amino acid composition: 
Ala (A)  18	  4.0%
Arg (R)  32	  7.2%
Asn (N)  24	  5.4%
Asp (D)  25	  5.6%
Cys (C)   5	  1.1%
Gln (Q)  12	  2.7%
Glu (E)  24	  5.4%
Gly (G)  23	  5.2%
His (H)  12	  2.7%
Ile (I)  28	  6.3%
Leu (L)  46	 10.3%
Lys (K)  35	  7.9%
Met (M)   5	  1.1%
Phe (F)  29	  6.5%
Pro (P)  29	  6.5%
Ser (S)  36	  8.1%
Thr (T)  16	  3.6%
Trp (W)  11	  2.5%
Tyr (Y)  18	  4.0%
Val (V)  17	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 67

Atomic composition:

Carbon      C	      2380
Hydrogen    H	      3647
Nitrogen    N	       647
Oxygen      O	       650
Sulfur      S	        10

Formula: C2380H3647N647O650S10
Total number of atoms: 7334

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    87570
Abs 0.1% (=1 g/l)   1.683, assuming all pairs of Cys residues form cystines


Ext. coefficient    87320
Abs 0.1% (=1 g/l)   1.678, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.72
This classifies the protein as unstable.



Aliphatic index: 79.98

Grand average of hydropathicity (GRAVY): -0.534